BD Canto II RUO Cytometer

描述（由申请人提供）：我们建议为斯坦福大学共享流式细胞仪设施购买Becton，Dickinson and Company生产的三激光Canto II研究专用细胞分析仪。该系统能够测量两个光散射信号和多达八个荧光信号，将取代设施工作人员在2000年定制的分析仪。定制的仪器现在为来自斯坦福大学35个不同研究实验室的大约120名用户提供服务。旧的分析仪变得越来越难以维护，部分原因是难以获得更换部件，并对正在进行的实验造成不可接受的故障风险。购买新仪器将确保共享流式细胞仪用户能够使用这一关键仪器。新的购买不会导致这些用户的充值费率增加。Canto II具有许多待更换仪器所不具备的功能。这些包括两个额外的荧光参数，562 nm激光激发和96孔板读数器。这些增加将使现有用户在规划实验时更加灵活，并将使设施高端LSR-II分析仪的现有用户能够选择使用较便宜的仪器。共用外地资产管制系统设施的工作人员对Canto使用的软件和电子设备有着丰富的经验，将其纳入共用外地资产管制系统设施，并培训现有用户群和新用户使用共用外地资产管制系统设施，应该不会有任何困难。在斯坦福大学共享流式细胞仪设施中进行的几乎所有工作都是与健康相关的研究，从分子生物学、遗传学和发展的基础研究到艾滋病和其他疾病的临床研究。该基金的大部分工作得到了NIH的支持。

DESCRIPTION (provided by applicant): We propose to purchase a three-laser Canto II research-only cell analyzer made by Becton, Dickinson and Company for the Stanford Shared FACS Facility. This system, which is capable of measuring two light scatter signals and up to eight fluorescence signals, will replace an analyzer custom built by Facility staff in 2000. The custom-built instrument now serves approximately 120 users from 35 different research labs at Stanford. The old analyzer is becoming increasingly difficult to maintain, in part due to difficulties in obtaining replacement parts, and poses an unacceptable risk of failure for ongoing experiments. Purchase of a new instrument will insure that Shared FACS Facility users have access to this critical instrumentation. The new purchase will not result in an increase in recharge rates to these users. The Canto II has a number of capabilities that are not available on the instrument to be replaced. These include two additional fluorescence parameters, 562-nm laser excitation, and a 96-well plate reader. These additions will allow current users enhanced flexibility in planning experiments and will provide current users of the Facility's high-end LSR-II analyzer the option of using a less-expensive instrument. The Shared FACS Facility staff has extensive experience with the software and electronics used in the Canto and should have no difficulties integrating it into the Facility and training the existing user base, as well as new users, in its operation. Almost all the work done in the Stanford Shared FACS Facility is health-related research, ranging from basic studies in molecular biology, genetics, and development to clinical research on AIDS and other diseases. The large majority of the work in the Facility is NIH supported.