Purchase of a Triple Quadrupole Mass Spectrometry System for Metabolite Analysis

描述（由申请人提供）：本提案寻求资金购买Agilent 6460三重四极杆（QqQ）质谱系统，该系统将由威尔康奈尔医学院（WCMC）的12名NIH支持的研究人员共享，用于生物分子的定量。该仪器满足了WCMC对小分子定量的关键未满足需求，并将通过纳入我们的MS核心设施而广泛提供给研究人员。值得注意的是，威尔康奈尔医学院MS核心设施自1997年以来一直在运作，并继续成为医学院及其附属医院（纽约长老会医院和特殊外科医院）MS仪器和支持的唯一来源。所要求的仪器将取代现有的三重四极杆MS，该MS现已使用13年，由于灵敏度差，无法用于我们的预期目的，源设计排除了与LC系统的接口，不能进行高度多路复用的多反应监测实验所需的快速转换，不能利用离子源和操作/该分析软件集成了我们现有的液相色谱和质谱系统，用于代谢物分析。所要求的仪器将支持威尔康奈尔对基础广泛的代谢物分析（靶向和非靶向）的坚定承诺，作为生物医学研究的转型工具。正如研究院院长随附的一封信所述，WCMC在财政上承诺维护该仪器，并在其整个使用寿命期间向NIH支持的生物医学科学家提供。 公共卫生相关性：此应用程序请求支持购买质谱仪，这将增强威尔康奈尔医学研究人员在NIH支持下的努力 大学扩大我们对人类健康和疾病的理解。申请人通过使用所要求的仪器瞄准的关键研究领域是癌症、传染病、干细胞生物学、糖尿病、动脉粥样硬化、结肠炎和神经生成。

DESCRIPTION (provided by applicant): This proposal seeks funds to purchase of an Agilent 6460 triple quadrupole (QqQ) mass spectrometry system that will be shared by 12 NIH-supported investigators at Weill Cornell Medical College (WCMC) for quantification of biomolecules. This instrument fulfills a critical unmet need at WCMC for small molecule quantification and will be made broadly available to investigators by inclusion in our MS core facility. Notably, the Weill Cornell Medical College MS core facility has been in operation since 1997 and continues to be the only source of MS instrumentation and support at the Medical College and its associated hospitals (New York Presbyterian Hospital and the Hospital for Special Surgery). The requested instrument will replace an existent triple-quadrupole MS that is now 13 years old and unusable for our intended purposes due to poor sensitivity, a source design that precludes interfacing with an LC system, inability to make the rapid transitions needed for highly-multiplexed multiple reaction monitoring experiments and inability to utilize ion sources and operating/analysis software that integrates our existing liquid chromatography and mass spectrometry systems for metabolite analyses. The requested instrument will support a solid commitment that Weill Cornell has made to broad-based Metabolite profiling, both targeted and untargeted, as a transformational tool for biomedical investigations. As documented in an accompanying letter from the Dean for Research, WCMC is financially committed to maintaining the instrument and making it available to NIH-supported biomedical scientists for its entire useful lifetime. PUBLIC HEALTH RELEVANCE: This application requests support for the purchase of a Mass Spectrometer that will enhance the efforts of NIH-supported researchers at Weill Cornell Medical College to expand our understanding of human health and disease. Key research areas that are being targeted by the applicants through use of the requested instrument are cancer, infectious diseases, stem cell biology, diabetes, atherosclerosis, colitis and neurogeneration.