CSHL Course on Yeast Genetics and Genomics

描述(由申请人提供)：冷泉港实验室酵母遗传学和基因组学课程建议继续在培训新使用酿酒酵母进行生物研究的研究人员方面发挥关键作用的悠久传统。课程名称反映了更加重视让学员熟悉基因组学和蛋白质组学中最新技术的使用。这门为期三周的高强度课程旨在让每个学生精通酵母遗传学的基础，然后为随着课程的进展而涵盖的更复杂的方法奠定概念基础。实验室实验分为12个部分，涵盖各种主题，包括：1.酵母工作的基础；细胞生物学中的经典遗传学和相关主题；2.酵母分子生物学简介；3.活细胞显微镜技术和基于图像的分析方法；4.遗传和物理相互作用分析；以及5.基因组学。这门课程的实验部分由讲师每天的理论讲座和世界级的研讨会系列组成，讲师因其在该系统中的成熟专业知识而被选中，并由使用酵母系统的知名研究人员举办一系列世界级研讨会。因此，每个学生都可以接触到他们所教授的方法的基本原理，以及该领域专家对这些方法的最新应用。这门课程有着良好的声誉，并寻求继续服务于不同的生物学家和物理科学社区；生物物理学、数学、化学、工程学、计算机科学。研究人员之所以被这门课程所吸引，是因为酵母的利用带来了明显的好处，因为这种模式生物中有大量可用的资源。此外，寻求将酵母菌方法纳入本科课程的教育工作者是该课程的临时学生。虽然这门课程的总体组织每年都保持不变，但随着酵母生物学和基因组学这一广泛领域的变化，课程内容自然发生了变化；随着新教师的更换(每年约1名)，新课程增加了新的专业知识，新教师将最新的技术和方法融入到课程中。由于每年至少保留两名教师，这种演变并不是以牺牲连续性为代价的。 与公众健康相关：酵母遗传学和基因组学暑期课程的主要目标是为学员提供有关酵母菌遗传学的经典、分子和基因组学方法的密集指导。本课程提供为期三周的密集实验室和讲座体验，为学员直接进入酵母遗传学和基因组学研究做好准备。

DESCRIPTION (provided by applicant): The Cold Spring Harbor Laboratory Yeast Genetics and Genomics Course proposes to continue with a long tradition of serving a critical role in the training of investigators new to the use of Saccharomyces cerevisiae for biological research. The course title reflects a greater emphasis on familiarizing participants with the use of the latest techniques in genomics and proteomics. This highly intensive three-week course is designed to make each student proficient in the fundamentals of yeast genetics, which then establishes the conceptual backbone for the more sophisticated methods that are covered as the course progresses. The laboratory experiments are broken into twelve multi-part sections that cover a variety of topics including: 1. Fundamentals of working with yeast; classical genetics and related topics in cell biology; 2. Introduction to yeast molecular biology; 3. Live cell microscopy techniques and image-based assays and analysis methods; 4. Genetic and physical interaction analysis; and 5. Genomics. The experimental portion of the course is complemented by daily theoretical lectures by the instructors chosen for their proven expertise in the system and a world-class seminar series from renowned investigators that utilize the yeast system. As a result every student is exposed to the underlying principles of the methods they are being taught and their state-of-the-art application by the experts in the field. The course has a well-established reputation for, and seeks to continue, serving a diverse community of biologists as well as the physical sciences; biophysics, mathematics, chemistry, engineering, computer science. Researchers are attracted to the course as a consequence of the clear benefit from the utilization of yeast, given the large number of resources available in this model organism. In addition, educators that seek to incorporate yeast approaches in undergraduate courses are occasional students in the course. Although the general organization of the course is intended to remain the same from year to year, there is a natural evolution in content as the broad field of yeast biology and genomics changes; as instructors are replaced (~1 per year) new expertise is added to the course and the latest technologies and approaches are incorporated into the curriculum by the new instructors. Since at least two instructors remain from year to year, this evolution does not come at the sacrifice of continuity. PUBLIC HEALTH RELEVANCE: The primary objective of the Yeast Genetics and Genomics summer course is to provide participants with intense instruction in classical, molecular and genomics approaches to the genetics of yeast. This course provides an intensive laboratory and lecture experience over a three week period that will prepare the participant to enter directly into research in yeast genetics and genomics.