ALTERED NUCLEAR TRANSFER - OOCYTE ASSISTED REPROGRAMMING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的目标是利用改变的体细胞核移植到灵长类动物的卵子中，希望产生多能干细胞，同时绕过在此过程中生产/破坏可行的胚胎。 我们发现，灵长类胚胎中的滋养外胚层谱系可以通过下调未受精卵母细胞中的CDX 2来废除。多能性内细胞谱系不受影响，并产生功能性ES细胞。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is utilization of altered somatic cell nuclear transfer into primate eggs with the hope of yielding pluripotent stem cells while bypassing the production/destruction of viable embryos in the process. We showed that the trophectodermal lineage in primate embryos can be abrogated by downregulation of CDX2 in unfertilized oocytes. The pluripotent inner cell lineage was not affected and produced functional ES cells.