OVARIAN TISSUE CRYOPRESERVATION IN NONHUMAN PRIMATES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目是路线图联盟（U 54 RR 02437，T。Woodruff，PI），题为“The Oncofertility Consortium：Fertility Preservation for Women”。其目标是提供领先的国家最先进的冷冻保存技术的从业者谁是保存人类卵巢组织的主持下，国家医师合作社。 具体目的是通过比较新型玻璃化冷冻-解冻方法与目前使用的缓慢冷冻方法，优化非人灵长类动物的卵巢冷冻保存方法，以恢复女性癌症幸存者的生育能力。 缓慢冷冻和玻璃化冷冻妇女卵巢组织导致怀孕后自体移植。然而，在许多癌症患者中，自体移植是禁忌的，因为有重新接种患者自身癌症的风险。在这组患者中，解冻后分离的次级卵泡的封装三维（3D）培养可能是产生成熟卵泡和可受精卵母细胞以保存生育力的最佳选择。 由于缓慢冷冻会损害次级卵泡，因此可以保存次级卵泡用于封装3D培养的冷冻/冷却方法将为患者在移植之外利用冷冻卵巢组织弥合技术差距。 卵巢组织保存的成功将包括评估a）剩余卵泡的组织学和免疫细胞化学特征; B）使用包封的3D培养物的次级卵泡存活、生长和功能特征;以及c）成熟卵母细胞的受精、早期胚胎发育和最终活后代的产生。 该项目是通过与西北大学生物材料核心的互动来促进的。 预期的进展将转移到生育中心的临床工作中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is part of a Roadmap Consortium (U54 RR02437, T. Woodruff, PI) entitled "The Oncofertility Consortium: Fertility Preservation for Women". The goal is to provide leadership in state-of-the-art cryopreservation technology to practitioners who are preserving human ovarian tissues under the aegis of the National Physicians Cooperative. The specific aim is to optimize ovarian cryopreservation methods in nonhuman primates for restoring fertility in female cancer survivors by comparing novel vitrification freeze-thaw methods to the currently utilized slow freeze method. Both slow freeze and vitrification of ovarian tissue in women have lead to pregnancies following autologous transplant. However, autologous transfer in many cancer patients is contraindicated because of the risk of re-seeding the patient with her own cancer. In this group of patients, encapsulated 3-dimensional (3D) culture of isolated secondary follicles post-thaw may be the best option for producing mature follicles and fertilizable oocytes for fertility preservation. Since slow freezing damages secondary follicles, a freezing/cooling method that could preserve secondary follicles for encapsulated 3D culture would bridge a technical gap for patients to utilize frozen ovarian tissue outside of transplantation. The success of ovarian tissue preservation will include assessment of a) histological and immunocytochemical characteristics of remaining follicles; b) secondary follicle survival, growth and functional characteristics using encapsulated 3D culture; and c) fertilization of the matured oocyte, early embryonic development, and ultimately production of live offspring. This project is facilitated by interactions with the Biomaterials Core at Northwestern University. Expected advances will be transferred to clinical efforts at the Fertility Centers.