STEM CELL BIOLOGY: NEW DIRECTIONS IN CLINICAL AND BASIC RESEARCH

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 COBRE有5个目标：1)发展一个强大的指导小组，由具有干细胞生物学、分子生物学、神经生物学和分化转录调控、肺生理学和生物学方面背景的成熟研究人员组成，并将基础研究转化为临床治疗。2)通过提供核心实验室、行政支持和总资源来加强基础设施支持，以提高教师的研究竞争力。3)招聘和保留有资金的初级和常设教员，以继续将RIH建立为主要的干细胞研究中心。4)确定骨髓干细胞的真实表型及其组织命运，研究损伤组织通过微泡传递表型信息，明确转录调控和分化机制。5)将基础干细胞研究转化为慢性病患者组织修复或矫正的临床试验，并将洞察力应用于癌症的治疗方法。一个经验丰富的指导小组将支持三个完整的项目和两个由初级调查人员领导的试点项目。这项工作围绕干细胞生物学和调节干细胞表型的方法进行了主题协调。目前的三个项目是：1)肺损伤及其对骨髓细胞表型的影响；2)酪氨酸磷酸酶Shp2在干细胞特性维持中的作用；3)转录调控和细胞信号事件的确定，这些事件塑造了多巴胺神经元前体细胞的分子特性，并确定了中脑多巴胺神经元的亚型。试点项目是：1)造血干细胞循环研究，2)人类诱导多能干细胞(IPSC)技术应用于脑部疾病。这些科学项目得到了行政、流式细胞仪和分子支持核心的支持。有持续监测的持续计划，初级PI每周向导师介绍情况，并为每个人逐步发展R01赠款制定明确的计划。目前正在招聘新的教员，这位科布雷显然促进了干细胞生物学中心的持续发展。这笔赠款在开发组织再生和癌症治疗的新方法方面，为扩大我们对干细胞生物学的理解带来了巨大的希望。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The COBRE has 5 objectives: 1) Development of a strong mentoring group of established investigators with complimentary backgrounds in stem cell biology, molecular biology, neurobiology, and transcriptional regulation of differentiation, pulmonary physiology, and biology, and the translation of basic research to clinical therapy. 2) The enhancement of infrastructure support by providing core laboratories, administrative support, and total resources to increase the research competitiveness of our faculty. 3) Recruit and retain funded junior and established faculty so as to continue the establishment of RIH as a major stem cell research center. 4) Determine the true phenotype of marrow stem cells and its tissue fate to study the transfer of phenotype information via microvesicles from injured tissue, and to define mechanisms of transcriptional control and differentiation. 5) Translate basic stem cell studies into clinical trials on tissue restoration or correction in patients with chronic diseases and apply insights into approaches to cancer. An experienced mentoring group will support three full projects and two pilots led by junior investigators. The work is thematically coordinated around stem cell biology in general and approaches to modulating the stem cell phenotype. The current three projects are: 1) Injured lung and its influence on marrow cell phenotype, 2) Tyrosine phosphatase Shp2 in stem cell property maintenance, and 3) Determining the transcriptional regulation and cell signaling events that shape the molecular identity of Dopamine neuron progenitors and specify subtypes of midbrain Dopamine neurons. The pilot projects are: 1) the study of cycling hematopoietic stem cells, and 2) human induced pluripotent stem cell (iPSC) technology applied to brain disease. The scientific projects are supported by the administrative, flow cytometry, and molecular supports cores. There are ongoing plans for continued monitoring with the junior PIs presenting weekly to the mentors, and with a defined plan for progressive development of R01 grants by each of the individuals. New faculty are currently being recruited, and this COBRE clearly facilitates continued development of The Center for Stem Cell Biology. The grant holds great promise for expanding our understanding of stem cell biology in developing new approaches for tissue regeneration and cancer therapy.