Vector Core

矢量核心在中心的当前周期内经历了实质性的演变， 在新型病毒载体方面取得了巨大的进展。Julie约翰斯顿博士，Vector Core总监 以她在DNA和RNA病毒载体方面的丰富经验监督核心。在过去的四年里， 从Vector Core提供的服务列表已呈指数级扩展。上一次的 该中心提供了基于腺病毒血清型5、腺相关病毒 病毒（AAV）血清型2和基于鼠的亲嗜性和亲嗜性逆转录病毒。目前 该中心提供的服务范围已扩大到包括：腺病毒-基于人类的载体 Ad 5和至少5种猴腺病毒，以及分离的更多新型猴腺病毒; AAV载体 基于人AAV血清型1、2和5以及猿AAV血清型7和8，该列表扩展到 包括10个新的AAV血清型在下一个周期的赠款;慢病毒-至少6个不同的 基于HIV和FIV的假型;和具有各种假型的鼠逆转录病毒。除此之外 增加载体生产，保证这些载体质量是非常重要的。目前有一种质量 对照组与载体核心，以检查在该单元中制备的任何载体制备物的同一性、效价和纯度。

The Vector Core has undergone substantial evolution during the current cycle of the Center to leverage the tremendous progress that has been made in novel viral vectors. Dr. Julie Johnston, Director of Vector Core oversees the core with her tremendous experience in DNA and RNA viral Vectors. During the last four years, the list of services provided from the Vector Core has expanded exponentially. At the time of the last renewal of the grant the core provided services with vectors based on adenovirus serotype 5, adenoassociated virus (AAV) serotype 2 and murine based ecotropic and amphotropic retroviruses only. Currently the list of services provided by the core has expanded to include: Adenovirus - vectors based on human Ad5 and at least 5 simian adenoviruses with more novel simian adenoviruses in isolation; AAV- vectors based on the human AAV serotypes 1,2 and 5 and simian AAV serotype 7 and 8 with this list expanding to include 10 additional novel AAV serotypes over the next cycle of the grant; lentiviruses - at least 6 different pseudotypes based on HIV and FIV; and murine retroviruses with various pseudotypes. In addition to this increase in vector production it was very essential to quality assure these vectors. Currently there is a quality control group with Vector Core to check identity, potency and purity of any vector prep made in this unit.