PRIMATE GENETIC ANALYSIS AND PEDIGREE MANAGEMENT

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 为了确定亲子关系，提取DNA的样本已经从2000多只恒河猴身上采集，这些猕猴是叶尔克的繁殖群体。这包括来自致力于艾滋病研究的SPF群体的动物，以及来自NSPF动物的动物。这些样本中的每一个都对分布在自体染色体上的19个高度多态的微卫星进行了基因分型。这些数据被用来确定我们在野外空间站维护的所有猕猴和黑尾猴的亲缘关系、系谱和选定的遗传标记。表征特定遗传成分的能力使我们能够更好地满足特定的研究人员的需求，开展更多样化的研究工作，有选择地为特定的遗传特征进行繁殖，并进行特定的表型比较。为了管理这个数据集，我们正在建立一个全面的数据库系统，它将能够同化所有动物的遗传、亲子关系、系谱和人口统计变量。这个数据库系统将被整合到我们目前的动物记录系统中，这将使耶克斯的调查人员和兽医能够在一个查询中跟踪、管理和查看动物的记录。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Samples for DNA extraction to determine parentage have been collected from over 2000 rhesus macaque, comprising the Yerkes' breeding colony. This includes animals from both the SPF colonies dedicated to AIDS research, and the animals from the NSPF animals. Each of these samples has been genotyped for 19 highly polymorphic microsatellites spread throughout the authosomal chromosomes. These data are used to determine parentage, pedigree, and selected genetic markers for all of our macaques and mangabeys maintained at the Field Station. The ability to characterize specific genetic components has enabled us to better meet specific investigator needs, to develop more diverse research endeavors, to selectively breed for specific genetic traits, and to undertake specific phenotypic comparisons. To manage this data set, we are establishing a full-scale database system that will be able to assimilate genetic, parentage, pedigree, and demographic variable on all the animals. This database system will be incorporated into our current animal records system, which will enable Yerkes' investigators and veterinarians to track, manage, and view an animal's record in a single query.