THE ROLE OF TIMP-2 IN NEURAL CREST PATHFINDING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 神经脊细胞从胚胎神经管中分离出来，然后在胚胎中广泛迁移，然后分化成一些衍生细胞。神经脊细胞的一个子集，称为心脏神经脊细胞，对心脏发育的几个方面做出贡献，包括间隔、心肌分化和流出道的正常形成。许多先天性心脏病与心脏神经脊细胞发育异常有关。然而，对胚胎中调控心脏神经脊迁移的因素知之甚少。我们已经证明，分泌蛋白TIMP-2在鸡心脏NC细胞中表达，是其正常发育所必需的。该项目测试了TIMP-2引导心脏NC细胞进入正确的迁移途径的假设，并将阐明其实现这一过程的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Neural crest cells detach from the embryonic neural tube and then migrate extensively throughout the embryo before differentiating into a number of derivatives. A subset of neural crest cells, called cardiac neural crest cells, contribute to several aspects of heart development, including septation, myocardial differentiation, and proper formation of the outflow tract. Many congenital heart defects are associated with abnormal cardiac neural crest cell development. However, very little is known about the factors that regulate cardiac neural crest migration in the embryo. We have shown the secreted protein TIMP-2 is expressed by chicken cardiac NC cells and required for their normal development. This project tests the hypothesis that TIMP-2 directs cardiac NC cells into the proper migratory pathway, and will elucidate the mechanism by which it does so.