STRUCTURE OF U6 SNRNA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们正在研究酿酒酵母U6单链RNA的结构。这个112个核苷酸的非编码RNA在真核生物进化过程中高度保守，形成了剪接体催化核心的一部分。已知U6单链RNA在前信使核糖核酸剪接过程中通过多种构象转换。RNA结构将在一系列温度、pH和离子条件下进行研究。我们需要收集的核磁共振实验包括：1D(1H)，2D NOESY，2D 1H-15N HSQC，2D 1H-15N HNN COSY，2D 1H-13C HSQC，2D NOESY，以及2D 1H，13C过滤/编辑NOESY。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are investigating the structure of U6 snRNA from S. cerevisiae. This 112 nucleotide non-coding RNA has been highly conserved throughout eukaryotic evolution, and forms part of the catalytic core of the spliceosome. U6 snRNA is known to transition through multiple conformations during pre-mRNA splicing. The RNA structure will be investigated at a range of temperatures, pH and ionic conditions. NMR experiments that we will need to collect include: 1D (1H), 2D NOESY, 2D 1H-15N HSQC, 2D 1H-15N HNN COSY, 2D 1H-13C HSQC, 2D NOESY, and 2D 1H,13C filter/edit NOESY.