FATTY ACID ANALYSIS OF GANGLIOSIDES BY GC-MS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 以Jurkat细胞+乙醇和Jurkat细胞+甘露醇神经节苷脂为原料，采用酸解法制备脂肪酸甲酯(FAME)。用14%的BF3在甲醇(Thermo Science)中，在100℃下进行1小时的甲醇分解。与以C19：0为内标的样品同时，以相同的方式对脂肪酸混合物参比标准品GLC 416(Nu-Chek Prep，Inc.)进行水解和酯化。用正己烷萃取FAME衍生物。用气相色谱-质谱仪(GC-MS)对其脂肪组成进行分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fatty acid methyl esters (FAME) were prepared from the Jurkat cell+ETOH and Jurkat cell+ManNDAz ganglioside samples by acid methanolysis. Methanolysis was performed with 14% BF3 in methanol (Thermo Scientific) at 100oC for 1 hr. A fatty acid mixture reference standard, GLC 416 (Nu-Chek Prep, Inc.), was hydrolyzed and esterified at the same time and in the same manner as the samples with C19:0 as internal standard. The FAME derivatives were extracted with hexane. Fatty composition analysis was performed by combined gas chromatography-mass spectrometry (GC-MS) of the FAME.