SIGNAL TRANSDUCTION COMPLEXES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们希望确定视觉效应酶PDE 6本身及其与其激活G蛋白转导素的复合结构。 环核苷酸磷酸二酯酶（PDE）是信号转导途径的关键调节组分，是当前药物和开发中药物的主要靶点。 没有结构可用于任何全长PDE同工酶，尽管有一些用于分离的结构域。 视觉G蛋白级联中的关键效应酶是cGMP特异性PDE 6，其遗传缺陷导致人类和动物失明。 它是一个200 kDa的异源四聚体（PDE 6？）。 它是一种脂化外周膜蛋白，与活化的G蛋白形成脂质依赖性复合物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We would like to determine the structure of the effector enzyme of vision, PDE6, by itself and in complex with its activating G-protein, transducin. Cyclic nucleotide phosphodiesterases, PDEs, are key regulatory components of signal transduction pathways, and are major targets of current drugs and those in development. No structures are available for any full-length PDE isozymes, although there are some for isolated domains. The key effector enzyme in the G protein cascade of vision is the cGMP-specific PDE6, genetic deficiencies in which cause blindness in humans and animals. It is a 200 kDa heterotetramer (PDE6????). It is a lipidated peripheral membrane protein, which forms a lipid-dependent complex with activated G-protein.