CELL MIGRATION CONSORTIUM PROJECT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 使用FCS确定迁移细胞中以下分子（按优先级顺序）的浓度、聚集状态和相互作用。（桩蛋白，α-辅肌动蛋白; FAK和桩蛋白; GIT 1和桩蛋白; GIT 1和Pix、Rac或Pak）。 通过FCS测定浓度和聚集度。 (1)粘连中和周围的分子数量;（2）异位表达，然后是内源性表达水平;（3）确定粘连中和周围的复合分子数量;（4）通过FCS检测可溶性组分的活化（paK，Rac）;（5）确定粘连形成和分散时聚集和相互作用的变化。并使用FCS确定单个和相互作用蛋白质的动力学

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Use FCS to determine the concentrations, aggregation states, and interactions of the following molecules (in priority order) in migrating cells. (paxillin, a-actinin; FAK and paxillin; GIT1 and paxillin; GIT1 and Pix, Rac, or Pak). Determine concentrations and degree of aggregation by FCS. (1) number of molecules in and around adhesions; (2) ectopic expression and then endogenous expression levels; (3) detrmine the number of complexed molecules in and around adhesions; (4) detect activation of soluble components by FCS (paK, Rac); (5) determine changes in aggregation and interactions as adhesions form and disperse. and determine dynamics of single and interacting proteins using FCS