CCR-Frederick Flow Cytometry Core

CCR-Flow Core是Frdereick国家癌症研究实验室的NCI和承包商研究人员不可或缺的资源。研究人员利用流动核心人员的专业知识和仪器，并由工作人员维护，以调查各种白细胞介素和肿瘤坏死因子在肿瘤生物学中的作用，以及炎症反应在肿瘤发生中的作用。另一个实验室正在研究肿瘤干细胞的基础生物学。其他实验室正在研究小鼠骨髓干细胞的分化机制。其他实验室正在研究自然杀伤细胞在识别人类白细胞抗原B中的作用，以及kir 3DS 1在HTLV-1感染中的作用。受过训练的调查员每天使用这些仪器，经常使用到深夜和周末。这为流量核心人员留出了更多时间，在今年前三季度对约550个样本进行分类，并对约7700个样本进行分析。如果没有调查人员获取数据和分析自己的样本，政府将不得不雇用全职经验丰富的个人来完成同样数量的工作。在过去的一年里，至少有19篇论文使用了从流动核心中获得的数据。 在过去一年中，为来自41名主要调查员的实验室的79名科学家个人生成了数据（或对样本进行了分类）。虽然CCR-Frederick流式细胞仪核心是CIP的一部分，但来自该项目的研究人员占该设施使用量的约27%。未来的目标包括通过将经过培训的研究人员队伍扩大到所有的CCR，从而扩大使用，利用核心的仪器和专业知识产生的科学的生产力和质量。这可以通过在LSRII Fortessa上增加一个405激光器，并用小型2激光6色仪器取代两个BD FACScan来实现。

The CCR-Flow Core is an indispensable resource for NCI and contractor investigators at the Frdereick National Lab for Cancer research. Investigators have used the expertise of the flow core staff and the instrumentation available and maintained by the staff to investigate the role of various interleukins and tumor necrosis factor in tumor biology and the role of the inflammatory response in tumorogenesis. Another lab is investigating the basic biology of tumor stem cells. Other labs are investigating mechanism of differentiation of mouse bone marrow stem cells. Other labs are investigating the role of natural killer cells in the recognition of human leukocyte anigen B and the role of kir3DS1 in HTLV-1 infection.The flow core staff has trained eighteen investigators in the first three quarters of the year to run their own samples. The instruments are used daily by the trained investigators often into the night and on the weekends. This leaves more time for the flow core staff to sort approximately 550 samples and analyze about 7700 samples in the first three quarters of this year. Without the investigators acquiring the data and analyzing their own samples, the government would have to hire full time experienced individuals to perform the same volume of work. At least 19 papers have been published in the past year using data obtained in the flow core. In the past year, data has been generated (or samples have been sorted) for 79 individual scientists from the laboratories of 41 principal investigators. Although the CCR-Frederick Flow Cytometry Core is part of the CIP, investigators from this program account for approximately 27% of the use of this facility.The future goals include keeping up with cutting-edge technology in the flow lab by expanding the pool of investigators trained to run and analyze their own samples to all of CCR in Frederick thereby expanding the use, productivity and quality of the science generated using the core's instrumentation and expertise. This could be achieved by adding a 405 laser to the LSRII Fortessa and replacing the two BD FACScans with small 2 laser 6-color instruments.