INBRE: URI: CENTRALIZED RES CORE FACILITY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 RI-INBRE集中研究核心设施为网络参与者和罗得岛生物医学研究界提供研究和培训支持已有八年。在当前资助期内，超过58个研究小组使用了该设施，平均季度累计使用频率为1，320次。该设施的用户包括32名教师，38名博士后研究员，90名研究生和25名本科生。设施工作人员向所有用户提供了个别培训和咨询，以便在各种研究项目中更好地利用现有技术。工作人员继续参与教学的各种技术在本科和研究生实验室课程由药学院提供。这些课程旨在为使用核心设施的学生提供理论和实践经验。此外，与制造商代表合作组织了关于使用Cellometer Vision和FLUOstar Optima多检测板读数器进行自动细胞计数和GFP转染效率的动手仪器培训讲习班。工作人员还举办了为期两天的分离科学讲习班，介绍如何使用HPLC和FPLC进行蛋白质纯化。向讲习班参加者提供了讲座、讨论和实践经验。 作为推广活动的一部分，该设施的工作人员访问了RI-INBRE支持的Salve Regina大学的教师和学生，并举办了题为“通过RI-INBRE集中研究核心设施可用于罗得岛州生物医学研究的技术”的研讨会。此外，罗杰威廉姆斯大学生物技术课程的学生和教师参观了核心设施。 会上展示了生物技术领域一些关键工具的使用情况。今年购置的新设备包括：Shimadzu AXIMA Performance MALDI-TOF-MS/MS质谱仪、SpectraMax-M2酶标仪、NanoDrop ND 2000 UV/维斯光谱仪和Thermo Multifuge离心机。MALDI-TOF-MS/MS的安装和培训定于2011年4月进行。新增的软件包括：GraphPad Prism Scientific Graphing和Canvas 12 Technical Illustration and Data Visualization。总共印刷了193张海报，只有非RI-INBRE研究人员支付适度的费用来支付消耗品的费用。继续免费向研究人员提供设备和咨询，使用率继续增加。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The RI-INBRE Centralized Research Core Facility is in its eight year of providing research and training support to the Network participants and the Rhode Island biomedical research community. During the current grant period more than 58 research groups have used the Facility, with an average quarterly cumulative frequency of 1,320 usages. The Facility users consisted of 32 faculty, 38 postdoctoral fellows, 90 graduate students, and 25 undergraduate students. Facility staff provided individual training and consultation to all users in order to better utilize the available technologies in various research projects. The staff continued to participate in teaching various techniques in the undergraduate and graduate laboratory courses offered by the College of Pharmacy. These courses are designed to provide both theoretical and hands-on experience to students using the Core Facility. In addition, hand-on instrument training workshops were organized in collaboration with the manufacturer's representative on automatic cell counting and transfection efficiency of GFP using Cellometer Vision and FLUOstar Optima multi-detection plate reader. The staff also conducted a two-day separation science workshop on the use of HPLC and FPLC for protein purification. Lectures, discussions and hands-on experience were provided to workshop participants. As part of the outreach initiative, the Facility staff visited RI-INBRE supported faculty and students at Salve Regina University and presented a seminar entitled "Technologies Available for Biomedical Research in the State of Rhode Island through RI-INBRE Centralized Research Core Facility". Also, students in a biotechnology course at Roger Williams University and the faculty instructor visited the Core Facility. The use of some of the key instruments in the field of biotechnology was demonstrated. New equipment acquired this year included the following: Shimadzu AXIMA Performance MALDI-TOF-MS/MS mass spectrometer, SpectraMax-M2 microplate reader, NanoDrop ND2000 UV/VIS spectrometer, and Thermo Multifuge centrifuge. Installation and training of MALDI-TOF-MS/MS is scheduled for April 2011. The software additions included: GraphPad Prism Scientific Graphing, and Canvas 12 Technical Illustration and Data Visualization. A total of 193 posters were printed, with only the non-RI-INBRE researchers paying a modest fee to cover the cost of consumables. The equipment and consultations were continued to be made available to the researchers at no cost and the usage has continued to increase.