Neural Aspects of Tinnitus

描述（由申请人提供）：本项目的总体目标是：(1)获得水杨酸盐和声学过度刺激引起的耳鸣的行为估计，并测量个体动物耳鸣的时间过程和音高；(2)识别神经活动的变化(单单位活动，(3)确定神经活动的时间过程和模式如何与耳鸣的音高和时间过程的行为测量相关联；(4)确定水杨酸诱发的耳鸣和噪声诱发的耳鸣的行为和神经生理指标是否可以被NS1883（一种钾通道激动剂，已显示出抑制水杨酸诱发的耳鸣的希望）抑制。将使用SIPAC和GPIAS两种行为技术来独立确定耳鸣的时间过程和音高。通过长期植入16通道电极阵列来评估清醒动物的自发和声音诱发的神经活动。该项目将显著提高我们对动物模型中噪音和药物诱导耳鸣的时间过程和音高的理解，有助于识别与耳鸣相关的听觉皮层关键神经生理变化，并评估钾通道激动剂抑制水杨酸盐和噪音诱导耳鸣的有效性。

DESCRIPTION (provided by applicant): The overall goals of this project are to (1) obtain behavioral estimates of tinnitus induced by salicylate and acoustic overstimulation and measure the time course and the pitch of the tinnitus in individual animals, (2) identify the changes in neural activity (single unit activity, local field potentials) in the auditory cortex of awake rats using16 channel recording electrodes and (3) determine how the time course and pattern of neural activity correlate with behavioral measures of the pitch and time course of tinnitus, (4) determine if the behavioral and neurophysiological measures of salicylate induced tinnitus and noise-induced tinnitus can be suppressed by NS1883, a potassium channel agonist that has shown promise in suppressing salicylate-induced tinnitus. Two behavioral techniques, SIPAC and GPIAS, will be used to independently confirm the time course and pitch of the tinnitus. Spontaneous and sound evoked neural activity will be assessed in awake animals using chronically implanted 16-channel electrode arrays. The project will significantly advance our understanding of the time course and pitch of noise and drug induced tinnitus in an animal model, aid in identifying the key neurophysiological changes in the auditory cortex that are associated with tinnitus, and evaluate the effectiveness of a potassium channel agonist in suppressing salicylate and noise-induced tinnitus.