Flow Cytometry

流式细胞术核心是一个收费设施，为密歇根大学综合癌症中心(UM-CCC)的成员提供流式细胞仪仪器和专业知识。Flow Core自1964年开始持续运作，自1997年起，主要设施位于综合癌症中心提供的专用实验室空间内，2010历年有95名UM-CCC附属成员使用该设施，占核心用户基础(187个用户总数)的51%，同时占Flow Core总使用量的82%。Flow Core还为密歇根大学的所有研究人员提供了广泛的基础和医学科学途径。 研究人员将经过预处理的样本送到Core进行流式细胞仪分析和/或细胞分类。核心以按服务收费的方式运营，充值率为120美元/小时。全面的癌症中心调查人员可以直接将50%的使用量计入CCC核心支持拨款。对于那些愿意在无人值守的仪器上提供自我操作的调查人员，充值率将进一步折扣。仪器由训练有素的流式细胞仪操作员(7.6 FTE)操作。使用该设施需提前预订(至少24小时)，先到先得。核心仪器包括一台Coulter MoFlo XDP多激光高速细胞分选仪、三台BD Bioscience FACSAria多激光高速细胞分选仪、两台BD Bioscience FACSDIVa多激光高速细胞分选仪、一台Coulter Cyan多激光分析仪、两台BD Bioscience FACSCanto II分析仪、两台BD Bioscience FACSCalibur多激光分析仪和一台Coulter Z2颗粒计数器。

The Flow Cytometry Core Core is a fee-for-service facility providing flow cytometry instrumentation and expertise to members of the University of Michigan Comprehensive Cancer Center (UM-CCC). The Flow Core has been in continuous operation since 1964 with the main facility residing in dedicated laboratory space provided by the Comprehensive Cancer Center since 1997, For calendar year 2010 there were 95 UM-CCC-affiliated members using the facility, accounting for 51% of the core user base (187 total users) while accounting for 82% of total flow core usage. The Flow Core also provides access to all UM investigators in a broad range of basic and medical sciences. Investigators deliver pre-processed samples to the Core for flow cytometric analysis and/or cell sorting. The core operates on a fee-for-service basis, with a recharge rate of $120/hour. Comprehensive Cancer Center Investigators are able to bill 50% of their usage directly to the CCC core support grant. Recharge rates are further discounted for those investigators willing to provide self-operation on unstaffed Instrumentation. Instruments are operated by trained flow cytometry operators (7.6 FTEs). Access to the facility Is reserved in advance (24-hour minimum) on a first-come basis. Core instrumentation includes one Coulter MoFlo XDP multilaser high-speed ceil sorter, three BD Biosciences FACSAria multilaser high-speed cell sorters, two BD Biosciences FACSDIVa multilaser high-speed cell sorters, a Coulter Cyan multilaser analzer, two BDBiosciences FACSCanto II analyzers, two BD Biosciences FACSCalibur multilaser analyzers, and a Coulter Z2 Particle Counter.