Imaging Core

我们最近获得了一笔资金，将一台过时的BioRad 600共焦显微镜更换为Leica TCS SP 光谱共聚焦显微镜（S10 RR 16708）。新的共焦的日常运行是在 先生Edward Doughnard是一位经验丰富的显微镜学家。道格的薪水目前部分由果蝇支付 计划项目赠款（N $44232），部分由Marder的N S 17813，部分由生物系基金。的 共聚焦是在使用费的基础上提供给所有研究者在布兰迪斯。目前，共焦显微镜被大量使用， （60-70小时/周），因为几个实验室（Marder、白色、Hall、Griffith）的项目高度依赖于 共焦，用于固定和处理组织的研究。 迫切需要额外的仪器和人员来满足我们用户群的需求。一 我们用户组的一个子集（Turrigiano [PI]、Marder、Griffith、Lisman和纳尔逊）申请了共享设备 资助购买徕卡光谱共聚焦/多光子显微镜，用于研究活体组织 电生理学（S10 RR 017938）。这笔赠款做得很好，有很好的机会得到资助。 该大学已承诺为安装这一仪器和进行翻修提供资金，但 目前没有为支助人员提供机构资金。艾德·道金斯先生将监督 新的共焦和多光子仪器，但这些新设备的日常运行将 需要一个额外的技术人员。我们也缺乏资金来购买电生理设备， 利用多光子装置的潜力。电生理学和多光子联合应用的可行性 对用户群体的成像将使我们能够在重要的新方向上扩展我们的研究计划。 我们的成像武器库中的第二个差距是缺乏用于延时成像的仪器和软件。一 我们用户组的许多项目需要多个荧光团的延时测量。设置成本 对于这种成像装置（操纵器、刺激器、培养室阶段）， 它不会在一个实验室中得到全职使用。增加用于此类分析的设备和软件 将扩展现有的项目，并允许用户调查这些新的，高度相关的研究途径。

We recently received funding to replace an obsolete BioRad 600 confocai microscope with a Leica TCS SP Spectral confocal microscope (S10 RR16708). The day-to-day running of the new confocal is under the direction of Mr. Edward Dougherty, an experienced microscopist. Dougherty's salary is currently paid in part by the Drosophila program project grant (N $44232), partly by Marder's N S 17813, and partly by Biology Department funds. The confocal is available on a fee-for-usage basis for all investigators at Brandeis. The confocal is presently heavily used (60-70 hours/week) as several laboratories (Marder, White, Hall, Griffith) have projects that intensively depend on the confocal, for the study of fixed and processed tissue. There is an urgent need for additional instrumentation and personnel to meet the needs of our user group. A subset of our user" group (Turrigiano [PI], Marder, Griffith, Lisman and Nelson) applied for a Shared Equipment Grant to purchase a Leica Spectral Confocal/Multiphoton microscope, to allow for the study of live tissue combhled with electrophysiology (S10 RR017938). This grant did very well and stands an excellent chance of being funded. The University has committed funds for the installation of this instrument and renovations to accommodate it, but there are currently no institutional funds available for support personnel. Mr. Ed Dougherty will provide oversight for the new confocal and the multiphoton instrument, but the day-to-day running of these new pieces of equipment will require an additional technician. We also lack funds to purchase the electrophysiology equipment needed to fully utilize the potential of the multiphoton set-up. The availability of combined electrophysiology and multiphoton imaging to the user group would allow us to extend our research programs in important new directions. A second gap in our imaging arsenal is the lack of instrumentation and software for time-lapse imaging. A number of the projects of our user group require time-lapse measurements of multiple fluorophores. The set-up costs for this imaging rig (manipulators, stimulators, culture chamber stage) have been prohibitive for any individual, since it would not receive full-time use in a single lab. The addition of equipment and software for this type of analysis would extend existing projects and allow users to investigate these new, highly relevant avenues of research.