Core Project

这是约翰·霍普金斯大学医学院听力与平衡中心为国家失聪和其他沟通障碍研究所的研究核心补助金提出的竞争性续签申请。该中心包括16名研究人员，他们来自耳鼻喉科--头颈外科、生物医学工程学、神经科学、神经病学、分子生物学和遗传学。这些实验室提供了20个R01奖项的研究基地，其中14个来自NIDCD，其科学目标从毛细胞的分子生物物理学到听力和平衡的行为分析。提出了三个研究核心，它们将促进正在进行的研究，并在现有计划之间的界面上刺激新的倡议。 1.工程核心将开发和分发先进的数据采集和信号分析软件，并为中心实验室提供一般硬件和软件支持。 2.组织学核心将为光学和电子显微镜研究提供技术援助和材料支持，包括为用户提供显微切割机和冷冻切割机。超薄切片和EM分析将由一名兼职的资深组织学家提供。此外，用户还将接受组织准备、切片和可视化技术方面的指导。荧光显微镜将通过位于核心的荧光显微镜和数字成像站使用。 3.神经生理学核心将开发和分发先进的CMS记录方法，用于行为自由的动物。将在核心工作人员的协助下实施和应用留置多电极阵列。 4.此外，一个行政股将监督各核心的业务，组织中心的发言者方案，提供文书和人事支助，并编写报告。

This is a competing renewal application from the Center for Hearing and Balance at Johns Hopkins University School of Medicine for a Research Core Grant from the National Institute for Deafness and Other Communication Disorders. The Center includes 16 investigators drawn from the Departments of Otolaryngology-Head and Neck Surgery, Biomedical Engineering, Neuroscience, Neurology, and Molecular Biology and Genetics. These laboratories provide a Research Base of 20 R01 awards, 14 of them from the NIDCD, with scientific aims ranging from the molecular biophysics of hair cells to behavioral assays of hearing and balance. Three Research Cores are proposed that will facilitate ongoing research and stimulate new initiatives at the interface between existing programs. 1. The Engineering Core will develop and distribute advanced data acquisition and signal analysis software, and provide general hardware and software support for Center laboratories. 2. The Histology Core will provide technical assistance and material support for light and electron microscopic studies, including access to microtomes and cryotomes for Users. Ultra-thin sectioning and EM analysis will be provided by a half-time senior histologist. Also, Users will receive guidance in tissue preparation, sectioning, and visualization techniques. Fluorescence microscopy will be available through a fluorescence microscope and digital imaging station in the Core. 3. The Neurophysiology Core will develop and distribute advanced CMS recording methods for use in freely-behaving animals. Indwelling multi-electrode arrays will be implemented and applied with the assistance of Core staff. 4. In addition, an Administrative unit will oversee operations throughout the Cores, organize the Center's speakers program, provide clerical and personnel support, and prepare reports.