Sensitive Triple Quadrupole Mass Spectrometer for Translational Research

描述(由申请人提供)：本申请是关于三重四极杆(QQQ)质谱仪系统的申请，目的是在国家犹太人健康中心(NJH)的质谱学设施内执行翻译和基础科学应用。该仪器将用于开发和进行高通量、使用多反应监测(MRM)的单链和多链分析，以便对各种生物液和复杂样品中的小分子和多肽(蛋白质)进行定量。在过去的4年里，国家犹太健康中心投入了近100万美元(不包括设备)，用于开发最先进的质谱学设施；这些技术是NJH超过25名研究人员研究的重要组成部分，他们的长期成功依赖于质谱学。该提案重点介绍了9个项目，这些项目的成功具体取决于能否获得所要求的文书。这些包括定量蛋白质测序，磷酸化事件的量化，以及临床分析的发展。我们的目标是继续为我们的研究人员提供共享的质谱学资源，以支持目前和拟议的由NIH资助的生物医学研究，NJH已为此投入了大量资源。设施中的仪器设备现在已有五年的历史，不再能满足我们调查人员的需要；我们正在采取措施维护一个最先进的设施，包括购置新的仪器。这项提案将具体展示 以下部分：1)对QQQ质谱仪的充分、合理的需求，2)操作和监督该仪器的现有专业知识，3)显著加强NIH资助的研究项目，4)实际的财务和管理计划，以及5)强有力的机构承诺。NJH的质谱学设施满足了我们由NIH资助的研究人员对最先进技术的巨大需求，以及对创新试点研究和临床诊断开发的巨大需求。申请的QQQ将是这一设施的重要组成部分。NJH通过每年25万美元的预算支持该设施，包括工资、维护和试剂。作为一家高度专注的机构，我们相信NJH准备在呼吸医学方面提供创新的、高影响的研究。只有在我们的调查人员仍然可以使用尖端技术的情况下，才能实现这一点。 公共卫生相关性：一项成功的应用将通过支持目前从事呼吸系统和免疫学研究的研究人员，对公共卫生产生重大影响。该仪器将用于支持有可能验证疾病候选生物标记物的研究，确定药物靶标，并提高我们对几种疾病基本机制的总体了解。

DESCRIPTION (provided by applicant): This application is for a triple quadrupole (QQQ) mass spectrometer system for the purpose of performing translational and basic science applications within the Mass Spectrometry Facility at National Jewish Health (NJH). The instrument will be used to develop and conduct high- throughput, single- and multi-plex assays using multiple reaction monitoring (MRM) in order to quantitate small molecules and peptides (proteins) from a variety of biofluids and complex samples. National Jewish Health has committed almost $1,000,000 (not including equipment) in the past 4 years towards the development of a state-of-the-art mass spectrometry facility; these technologies are an essential component of research for over 25 investigators at NJH whose long-term success is dependent on mass spectrometry. This proposal highlights 9 projects whose success depends specifically on the acquisition of the requested instrument. These include quantitative protein sequencing, quantitation of phosphorylation events, and clinical assay development. Our objective is to continue to provide our investigators with a shared mass spectrometry resource to support current and proposed NIH-funded biomedical research and NJH has committed substantial resources towards this end. Instrumentation in the facility is now five years old and no longer adequate to the needs of our investigators; we are taking steps to maintain a state-of-the-art facility, including acquisition of new instruments. This proposal will specifically demonstrate the following components: 1) a substantial, justified need for a QQQ mass spectrometer, 2) existing expertise to operate and oversee the instrument, 3) significant enhancement of NIH-funded research projects, 4) a practical financial and management plan, and 5) a strong institutional commitment. The NJH mass spectrometry facility addresses the tremendous demand for state-of-the-art technologies by our NIH-funded researchers as well as for innovative pilot studies and the development of clinical diagnostics. The requested QQQ will be an essential component of this facility. NJH supports the facility through an annual budget of $250,000, including salary, maintenance, and reagents. As a highly focused institution, we believe that NJH is poised to deliver innovative, high-impact studies in respiratory medicine. This can only be achieved if cutting edge technologies remain available to our investigators. PUBLIC HEALTH RELEVANCE: A successful application will have a significant impact on public health by supporting researchers who are currently engaged in respiratory and immunological research. The instrument will be used to support studies where there is the potential to validate candidate biomarkers for disease, identify drug targets, and improve our overall knowledge of the basic mechanisms of several diseases.