Improved methodologies to measure novel alcohol biomarkers in whole blood

描述(申请人提供)：磷脂酰乙醇(PEH)是一种仅在乙醇存在下通过磷脂酶D作用形成的磷脂。由于其高特异性和低降解率，已在全血中被检测并被建议作为酒精滥用的标志。酗酒者血液中苯丙氨酸的平均半衰期为4天，在戒酒2周后仍可测得。我们的总体目标是扩大我们成功的前期工作，并开发一种灵敏的、高通量的用于全血中PEH分析的ELISA生物标志物分析方法。目前多环芳烃的分析方法主要基于高效液-质联用法。无法进行快速检测以及检测缺乏标准化，阻碍了这些生物标记物的临床应用。这一应用将产生最终的peth酶联免疫吸附试验产品，可能对酒精使用障碍的研究和管理产生重大影响。 公共卫生相关性：我们的总体目标是在我们成功的前期工作的基础上扩大我们的工作范围，并开发一种灵敏的、高通量的用于全血中多酚类药物分析的ELISA生物标记物分析方法。

DESCRIPTION (provided by applicant): Phosphatidylethanol (PEth) is a phospholipid formed only in the presence of ethanol via the action of phospholipase D. It has been measured in whole blood and proposed as a marker of ethanol abuse due to its high specificity and low degradation rate. The mean half-life of PEth in blood from alcoholics was found to be 4 days, and PEth was still measurable after 2 weeks of sobriety. Our overall goal is to expand on our successful preliminary work and develop a sensitive, high throughput ELISA biomarker assay for PEth analysis in whole blood. Current methods of analysis of PEth are based on the high performance liquid chromatography-mass spectrometric methods. Unavailability of rapid assays for the detection and the lack of standardization of the tests have hampered the clinical use of these biomarkers. The final PEth ELISA product that would result from this application could have a significant impact in the study and management of alcohol use disorders. PUBLIC HEALTH RELEVANCE: Our overall goal is to expand on our successful preliminary work and develop a sensitive, high throughput ELISA biomarker assay for PEth analysis in whole blood.