LUMINEX TECHNOLOGY FOR THE QUANTIFICATION OF CYTOKINES IN NON-HUMAN PRIMATES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 非人灵长类动物（NHP）是临床前研究的首选动物模型，因为它们在生理学和遗传学上比任何其他动物更接近人类。 通过使用NHP模型，免疫学研究取得了重大进展，最显著的是在艾滋病发病机制、治疗和疫苗开发方面。 细胞因子和趋化因子是免疫系统的可溶性介质，其在细胞间信号传导和将细胞募集到炎症部位中起关键作用。 NHP中这些分子的鉴定对于理解这些物种中发生的复杂生理和病理机制以及证明这些机制在人类中是否具有相似的功能至关重要。 Luminex 100系统是一款台式流式细胞仪，允许用户在单个试管中同时定量多达100个分子。 最近，我们鉴定了几种特异于人细胞因子的抗体，其具有识别同源趋化因子和NHP来源的细胞因子的能力。 目前，我们有一组试剂，可以同时鉴定23种细胞因子和趋化因子，这些细胞因子和趋化因子来自黑猩猩和旧世界猴恒河猴、狒狒、食蟹猴、猪尾猕猴和非洲绿色猴。 在该R-24应用中，我们提出通过完成以下具体目标来促进和扩展Luminex技术在NHP中的使用：1）通过增加旧世界猴物种中可检测分子的数量来提高我们的23重NHP Luminex板的广度和分析能力; 2）通过增加目前用于定量来自旧世界猴物种的细胞因子的一些次优试剂的灵敏度来提高我们的23重NHP Luminex面板的质量和深度;和3）开发用于同时定量绒猴（marmoset）中的细胞因子的重组抗体，绒猴是一种新的世界猴物种，越来越多地用于疫苗开发、衰老和肥胖研究。 我们将对现有的抗旧大陆猴细胞因子的单克隆抗体进行广泛的测试，并采用最先进的重组抗体技术来制备抗绒猴细胞因子的抗体。 这些研究产生的信息和方案将在SNPRC的流式细胞术网站上列出;新抗体将分发给在成本回收基础上证明其需要的科学家。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Nonhuman primates (NHP) are the preferred animal models for pre-clinical research because they approximate humans in physiology and genetics more closely than any other animal. Critical advances in immunologic research have been made through the use of the NHP model, most notably in aids pathogenesis, treatment, and vaccine development. Cytokines and chemokines are soluble mediators of the immune system that play a crucial role in intercellular signaling, and in the recruitment of cells to inflammation sites. Identification of these molecules in NHP is crucial for the understanding of complex physiological and pathological mechanisms that occur in these species, and to demonstrate whether these mechanisms function similarly in humans. The luminex100 system is a bench-top flow cytometer that allows the user to quantify up to 100 molecules simultaneously in a single tube. Recently, we identified several antibodies specific for human cytokines that have the capacity to recognize homologous chemokines and cytokines of NHP origin. Currently, we have a panel of reagents that allow for the simultaneous identification of 23 cytokines and chemokines from chimpanzees and the old world monkeys rhesus macaques, baboons, cynomolgus macaques, pig-tailed macaques, and African green monkeys. in this r-24 application we propose to facilitate and expand the use of the Luminex technology in NHP by completing the following specific aims: 1) to improve the breadth and analytical power of our 23-plex NHP Luminex panel by increasing the number of detectable molecules in old world monkey species; 2) to improve the quality and depth of our 23-plex NHP Luminex panel by increasing the sensitivity of some suboptimal reagents currently used for quantification of cytokines from old world monkey species; and 3) to develop recombinant antibodies for the simultaneous quantification of cytokines in the marmoset, a new world monkey specie increasingly used in vaccine development, aging, and obesity research. We will perform extensive testing of available monoclonal antibodies against old world monkey cytokines, and state-of-the-art recombinant antibody technology for the creation of antibodies against marmoset cytokines. The information and protocols generated from these studies will be listed in the flow cytometry website of the SNPRC; novel antibodies will be distributed to scientists who justify their need on cost-recovery bases.