THE ROLE OF ACTIVITY IN SCULPTING NEURONAL FORM AND FUNCTION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这个项目将研究活动在控制神经元的兴奋性和形态方面的作用。我们已经成功地制造了条件性小鼠，在这种小鼠中，我们可以以细胞自主的方式，通过删除所有AMPA和NMDA受体来沉默所有对该神经元的兴奋性输入。然后可以在沉默后的不同时间间隔对这些神经元进行研究，以检查对神经元兴奋性和形态的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project will examine the role of activity in controlling the excitability and morphology of neurons. We have succeeded in making conditional mice in which we can, in a cell autonomous manner, silence all excitatory input to that neuron, by deleting all AMPA and NMDA receptors. These neurons can then be studied over various time intervals after silencing to examine the effects on neuronal excitability and morphology.