ENGINEERING METHYLTRANSFERASE INVOLVED IN THE BIOSYNTHESIS OF AT2433

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 甲基转移酶是参与天然产物生物合成的一种剪裁酶。通过工程改造MT以转移甲基以外的基团将具有产生化学治疗剂的潜力。在这个项目中，我们希望进行与合成辅因子的结合研究，以使酶混杂。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Methyltransferases (MTs) are one of the tailoring enzymes involved in the biosynthesis of natural products. By engineering the MTs to transfer groups other than methyl will have the potential to generate chemotherapeutics. In this project, we wish to carry out binding studies with synthetic cofactors in order to make the enzyme promiscuous.