OPTICAL CLEARING OF HUMAN SKIN: IN VITRO AND IN VIVO STUDIES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 人体皮肤的光学透明化.体外和体内研究的初步结果 背景：皮肤是一个高度散射的结构，由于其复杂的形态。基于光的诊断和治疗程序由于散射而损失大量的光。诊断和治疗过程中缺乏足够的穿透力通常通过增加入射光的功率和辐射曝光来补偿。这些措施通常会导致不希望的副作用，如表皮和真皮损伤，导致感染、起泡甚至疤痕。因此，减少皮肤散射对于更好地穿透完整的人类皮肤是期望的。 材料和方法：创建不同浓度的聚丙二醇和聚乙二醇的化学组合。使用全厚度人类皮肤在体外测试了多种化学品的光学清除潜力。还通过使用光学相干断层扫描和测量非侵入性成像的最大深度，在体内测试了各种化学品的光学清除潜力。还使用局部施用的光学透明剂在体内测试了基于光的治疗程序，例如激光辅助脱毛、纹身去除和葡萄酒色斑的激光治疗，作为在Beckman激光诊所正在进行的IRB批准的研究的一部分。 结果：特定比例的PPG和PEG的化学组合在体外以及体内产生显著的皮肤清洁。OCT成像的最大皮肤深度显著增加。当与局部应用的清除剂组合时，基于光的治疗程序产生显著更少的表皮和真皮副作用。此外，更好的光穿透治疗程序也减少了治疗的数量需要达到预期的结果。 结论：局部施用的光学透明剂减少了光的表面以及皮肤散射，因为它在体外和体内穿透完整的人皮肤。如果在期望的过程之前进行皮肤的光学清洁，则基于光的诊断以及治疗过程可以显著地受益。此外，散射的减少提供了表皮以及真皮保护，从而降低了显著副作用如感染、色素变化、起泡和瘢痕形成的发生率。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Optical clearing of human skin; preliminary results of in vitro and in vivo studies Background: Skin is a highly scattering structure due to its complex morphology. Light-based diagnostic and therapeutic procedures lose a significant amount of light due to scattering. Lack of adequate penetration for diagnostic and therapeutic procedures is often compensated by increasing the power and radiant exposure of the incident light. Such measures often lead to un-desirable side effects such as epidermal and dermal injury leading to infection, blistering and even scarring. Reduction in skin scattering is therefore desirable for better light penetration thru intact human skin. Material and methods: chemical combinations of different concentrations of polypropylene glycol and polyethylene glycol were created. Optical clearing potential of a variety of chemicals was tested in vitro, using full thickness human skin. The optical clearing potential of various chemicals was also tested in vivo, by using optical coherence tomography and measuring the maximum depth imaged non-invasively. Light-based therapeutic procedures such as laser assisted hair removal, tattoo removal and laser treatment of port-wine stains was also tested in vivo, using the topically applied optical clearing agents, as part of an IRB approved study on-going at Beckman laser clinic. Results: chemical combinations of PPG and PEG in a specific ratio produced significant clearing of the skin in vitro as well in vivo. The maximum depth of skin as imaged with OCT was markedly increased. Light-based therapeutic procedures produced significantly less epidermal and dermal side effects when combined with the topically applied clearing agent. Additionally, better light penetration for therapeutic procedures also reduced the number of treatments required to achieve the desired result. Conclusions: topically applied optical clearing agents reduce surface, as well as dermal scattering of light, as it penetrates intact human skin in vitro and in vivo. light-based diagnostic as well as therapeutic procedures can benefit significantly if optical clearing of the skin is performed prior to the desired procedure. Additionally, reduction in scattering provides epidermal as well as dermal protection, thereby reducing the incidence of significant side effects such as infection, pigmentary changes, blistering and scarring.