IN-VIVO SKIN IMAGING

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目的主要目标是开发一种强大的人类皮肤成像的非线性显微镜方法。 这将包括激发/发射参数优化、成像深度改进（即皮肤的光学透明、目标探测器灵敏度）和运动伪影预防。 成像目标是双重的。首先，更多细胞的局部皮肤层（形成50-75 μ m皮肤的角质层和表皮）承受皮肤癌发生的主要冲击：黑素细胞位于基底表皮层内，表皮负责基底和鳞状细胞癌以及良性病变（光化性角化病，皮脂腺角化病等）的发生。这是保护身体免受环境因素如微生物、湿气、UV等影响的第一屏障。 下层真皮（取决于身体部位，厚度为2-4 mm）由结缔组织、弹性和胶原纤维的ECM构成，其细胞组分负责伤口愈合和免疫反应等。 人的皮肤反映了年龄、性别、种族、健康状况。具有显微分辨率和光谱选择性的皮肤成像可以为理解伤口愈合、致癌、衰老和环境响应的重要生物学和生物力学过程提供有力的工具。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The main goal of this project is to develop a robust method of non-linear microscopy of human skin imaging. This will include the excitation/emission parameters optimization, depth of imaging improvement (i.e. optical clearing of skin, objective-detector sensitivity) and motion artifacts prevention. The imaging goals are two-fold. First, the more cellular topical skin layers (stratum corneum and epidermis that form fist 50-75 ¿m of skin) are bearing the main brunt of skin carcinogenesis: melanocytes are localized within the basal epidermal layer, epidermis is responsible for basal and squamous cell carcinoma as well as benign lesions (actinic keratosis, sebhorreic keratosis, etc) genesis. This is a first barrier protecting the body from environmental factors such as microorganisms, moisture, UV, etc. The underlaying dermis (2-4 mm thickness depending on the body part) bulk is made of connective tissue, a ECM of elastic and collagen fibers, cellular component of which is responsible for wound healing and immune response among others. Human skin reflects age, gender, race, health status. Imaging skin with microscopic resolution and spectral selectivity can provide a powerful tool in understanding important biological and biomechanical processes of wound healing, carcinogenesis, aging, and environmental response.