FREEZING 70S CRYSTALS FROM THERMUS THERMOPHILUS UNDER PRESSURE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 为了定位与70年代核糖体结合的小的临床相关药物，我们正在努力收集尽可能高分辨率的衍射数据。在我们的实验中，我们注意到我们的晶体对它们被冻结的方式非常敏感。我们希望，在压力下将晶体冻结在液氮中将使我们能够获得更高质量的衍射数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In order to localize small clinical relevant drugs bound to the 70S ribosome, we are striving to collect the highest resolution of diffraction data possible. During our experiments we noticed that our crystals are very sensitive to the way they are frozen. We are hoping that freezing our crystal in liquid nitrogen under pressure will allow us to gain higher quality of diffraction data.