COMPARATIVE PROTEOMICS AND GLYCOPROTEOMICS OF PROTEINS SECRETED FROM B CINEREA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 灰霉病菌是一种广泛存在的植物病原菌，通过在寄主组织上引起灰霉病或软腐病，影响许多蔬菜和水果以及大量的灌木、树木、花卉和杂草。灰霉病菌主要侵染双子叶植物和非禾本科单子叶植物，不仅发生在作物的生长期，也发生在收获产品的贮藏过程中。因此，它要对重大的经济损失负责。大量的研究集中在了解灰霉菌的感染过程，这反过来又指导了有效预防策略的发展。灰霉菌在感染过程中必须突破细胞壁，从而分泌大量能降解细胞壁的酶。关于这些酶的特性，人们知之甚少。这些细胞壁降解酶中的大多数是糖基化的。尽管糖基化对蛋白质的结构和活性具有重要作用，但对几种重组蛋白的糖基化反应却知之甚少。为此，我们已经开始对这种真菌的分泌蛋白质组和糖蛋白质组进行鉴定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The fungus Botrytis cinerea is a widespread plant pathogen that affects many vegetables and fruits, as well as a large number of shrubs, trees, flowers, and weeds by causing grey mold or soft rot on the host tissues. B. cinerea mainly infects dicotyledons and non-graminaceous monocotyledons, and the infection not only occurs during the growth phase of crops but also during storage of the harvested products. Thus, it is responsible for significant economic losses. A great deal of research has focused on understanding the B. cinerea infection process, which in turn directs the development of effective prevention strategies. B. cinerea must breach the cell wall during the infection process and accordingly secretes a great number of enzymes that can degrade cell walls. Little is known concerning the identity of these enzymes. The majority of these cell wall degrading enzymes are glycosylated. Although the glycosylation on several recombinant proteins have been characterized, very little is known on the native enzymes, despite the importance of glycosylation on protein structure and activity. To this end, we have started to characterize the secreted proteome and glycoproteome of this fungus.