PROFILING OF C60 FULLERENE SAMPLES BY MALDI-TOF-TOF MS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 研究方法： 基质辅助激光解吸飞行时间质谱（MALDI-TOF/TOF MS） 将所有样品用甲苯溶解（样品4和5在水中，因此首先冻干），直接结晶到无基质的MALDI板中（Lee等人，2009），干燥并在反射器正离子模式下分析。 预期样品1和2在其整体结构中含有羧酸（客户提供的信息），因此，将样品1和2再干燥并用氯仿：甲醇（2：1）再溶解，如前所述结晶至MALDI板中，并以反射器负离子模式进行分析。 使用AB SCIEX TOF/TOF 5800（Applied Biosystem MDS Analytical Technologies）通过MALDI-TOF/TOF-MS进行富勒烯的分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Methods: Profiling by Matrix-Assisted Laser-Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF/TOF MS) All samples were dissolved with toluene (Samples 4 and 5 were on water, hence, lyophilized first), crystallized directly into a MALDI plate without matrix (Lee et al. 2009), allowed to dry and analyzed in reflector positive ion mode. Samples 1 and 2 were expected to contain carboxylic acid in their overall structures (information provided by the client), hence, were redried and redissolved with chloroform:methanol (2:1), crystallized into a MALDI plate as previously described and also analyzed in reflector negative ion mode. Analysis of fullerenes was performed by MALDI-TOF/TOF-MS using AB SCIEX TOF/TOF 5800 (Applied Biosystem MDS Analytical Technologies).