PROTON NMR OF FIVE OLIGOSACCHARIDE SAMPLES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 NMR光谱 通过溶解在D2 O中并冻干来对样品进行氘交换。将样品溶解在80 μ L D2 O中，加入5 μ L 1%丙酮的D2 O溶液作为内标，并将样品置于3 mm Shigemi NMR管中。在Varian Inova-600 MHz光谱仪上在25 ℃下获得1-D质子、TOCSY和NOESY NMR光谱，用水预饱和运行，以及梯度增强COSY和HSQC光谱。 测量相对于内部丙酮的化学位移（H=2.218 ppm，C=33.00 ppm）。 使用标准Varian采集参数;表1中列出了一些可变参数。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. NMR Spectroscopy The samples were deuterium exchanged by dissolving in D2O and lyophilization. The samples were dissolved in 80 ¿L D2O, 5 ¿L 1 % acetone in D2O was added as internal reference, and the samples were placed in a 3 mm Shigemi NMR tube. 1-D Proton, TOCSY and NOESY NMR spectra, run with water presaturation, and gradient enhanced COSY and HSQC spectra were acquired on a Varian Inova-600 MHz spectrometer at 25 ¿C. Chemical shifts were measured relative to internal acetone (¿H=2.218 ppm, ¿C=33.00 ppm). Standard Varian acquisition parameters were used; some variable parameters are listed in Table 1.