STRUCTURAL STUDIES IN RHODOPSIN ACTIVATION AND RPE65

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 视觉信号是一个可访问的范例，而在理解视觉和致盲疾病的极端重要性，也是一个可访问的范例，用于理解的大分子机制，所有G蛋白偶联受体信号事件的基础。我们利用光活化的视紫红质及其复合物，通过生物化学、生物物理学和结构研究的组合来探测类似于GPCR的活化状态的视紫红质的光态。RPE 65是负责视觉所需的发色团再生的异构酶，并且与几种人类致盲疾病有关。通过在NECAT和BNL的X29进行的工作，我们在2009年解决了这种蛋白质的第一个结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Visual signaling is an accessible paradigm while of extreme importance in understanding vision and blinding diseases is also an accessible paradigm for the understanding of the macromolecular machinery that underlies all G protein coupled receptor signaling events. We utilize photoactivated rhodopsin and complexes thereof to probe photostates of rhodopsin that are analogous to activated states of GPCRs through a combination of biochemistry, biophysics and structural study. RPE65 is the isomerase responsible for the regeneration of the chromophore necessary for vision and has been implicated in several human blinding disorders. Through work performed at NECAT as well as at X29 at BNL we solved the first structure of this protein in 2009.