ENDOCYTOSIS AND CELL CYCLE IN SACCHAROMYCES CEREVISIAE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 最近的活细胞成像研究，加上越来越多的实验性遗传学， 生物化学和药理学数据扩大了我们的分子知识， 内吞作用期间发生的事件和肌动蛋白所起的作用（Toret和Drubin， 2006年）。在这次合作中，我们的目标是发展更深入的了解内吞作用， 酵母使用相关荧光和软X射线断层成像的细胞 许多不同的突变体内吞表型的超微结构。的信息 不仅突出了这些突变对内吞过程的影响， 和机械，但确定这些变化对组织的影响， 整个细胞。细胞周期是所有生物细胞 从单细胞细菌到多细胞哺乳动物的生物体复制。最 细胞周期的基本功能是精确复制细胞中大量的DNA。 染色体，然后将拷贝精确地分离成遗传上相同的 子细胞它已被证明，有一个一般的控制，保持一个比例， 核体积与细胞体积的比值。然而，在整个细胞周期中， 尚未完全阐明。此外，了解其他人如何 细胞器是在细胞周期中遗传的。此次合作的另一个目标是利用 软X射线断层扫描以研究细胞器定位和体积的变化， 细胞周期各阶段细胞器的密度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent live-cell imaging studies, coupled with a growing body of experimental genetic, biochemical and pharmacological data have expanded our knowledge of the molecular events that occur during endocytosis and the role played by actin (Toret and Drubin, 2006). In this collaboration we aim to develop a deeper understanding of endocytosis in yeast using correlated fluorescence and soft x-ray tomography to image the cellular ultrastructure of a number of different mutant endocytic phenotypes. The information obtained will not only highlight the effect of these mutations on the endocytic process and machinery, but establish the consequences of these changes on the organization of the entire cell. The cell cycle is the essential mechanism by which the cells of all living organisms from unicellular bacteria to the multicellular mammals duplicate. The most basic function of the cell cycle is to accurately duplicate the vast amount of DNA in the chromosomes and then segregate the copies precisely into genetically identical daughter cells. It has been shown that there is a general control that maintains a ratio of nuclear volume to cell volume. However, such correlation during the entire cell cycle has not been fully elucidated. In addition, it is very important to understand how other organelles are inherited during the cell cycle. Another goal of this collaboration is to use soft xray tomography to study the changes in organelle positioning and the volume and density of organelles during the phases of the cell cycle.