NEW SUBSTRATES FOR FLUORESCENCE MICROSCOPY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们在这个项目中的目的是设计一个易于实现的方案，荧光显微镜具有高的空间分辨率和荧光增强性能的基础上共振激发的2D-PhC导模。 众所周知，外部辐射与周期性纳米结构（诸如PhC）的相互作用影响电磁波的远场传播。简而言之，周期性允许近场区域中的倏逝波与由PhC支持的局部泄漏模式的相位匹配。因此，泄漏模式的外部激发与谐振波长的全反射现象相关联。然而，光不会突然反射;并且在PhC和介质（通常是空气或水）之间的界面上存在被称为倏逝波区域的照明区域，其中强度从PhC表面呈指数衰减。使用该PhC的性质和先前显示的荧光增强[Estrada等人，2010. Optics Express，Vol. 18，Issue 4，pp. 3693-3699（2010）]，我们正在设计用于荧光显微术的新基底。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our purpose in this project is to design an easy-to-implement scheme for fluorescence microscopy with high spatial resolution and fluorescence enhancement properties based on the resonant excitation of 2D-PhC guided modes. It is well known that the interaction of an external radiation with a periodically nanostructure such as a PhC affects the far field propagation of electromagnetic waves. In brief, the periodicity allows for phase-matching of evanescent waves in the near field region to localized leaky modes supported by the PhC. Thus, the external excitation of leaky modes is associated with a total reflection phenomenon for a resonant wavelength. However, the light does not reflect abruptly; and an illuminated region called the evanescent wave region, is present on the interface between the PhC and the medium (typically air or water) where the intensity decays exponentially from the PhC surface. Using this PhC's property and the fluorescence enhancement shown previously [Estrada et al, 2010. Optics Express, Vol. 18, Issue 4, pp. 3693-3699 (2010)] we are designing new substrates for fluorescence microscopy.