DIFFUSION IN THE C ELEGANS GERM LINE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 梭线虫生殖细胞系形成合胞体，使得调节蛋白可以从一个细胞扩散到另一个细胞。我们使用单点荧光相关光谱和配对相关来量化扩散特性。这些定量数据将被用来建立数学模型，解释生殖干细胞如何决定自我更新或分化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The C. elegans germ line forms a syncytium, making it possible for regulatory proteins to diffuse from one cell to another. We are quantifying diffusion properties using single-point fluorescent correlation spectroscopy and pair-correlation. Those quantitative data will be used to formulate mathematical models explaining how germline stem cells make a decision to self-renew or to differentiate.