Analytical & Phenotyping Core

分析和表型核心的目的是提供交感神经压力反射功能的最先进的心血管和生化分析，包括交感神经交通、儿茶酚和去甲肾上腺素清除和溢出的测量。这一资源对我们小组识别和表征涉及自主神经系统的新疾病（如多巴胺-β-羟化酶缺乏症）至关重要。在本核心中建立了各种技术来支持各个项目，包括血浆容量（项目1和4）、心率和血压变异性（项目1、2、3和4）以及压力反射功能（项目1和4）的测定。 特别是项目2，其中所有其他将利用交感神经活动的生化评价，补充临床核心中详述的其他自主评价方法。将在项目1、3和4中利用用于表型分析的心血管参数超日节律的专业知识。 项目1和4将使用我们的新信号处理工具来处理神经信号。此外，我们还实施了一种动物表型分析设施，将开发用于评估人类自主神经功能的方法适用于将主要用于项目1的小鼠。这尤其包括监测清醒的无限制小鼠中的动脉内血压，监测完整和去窦弓神经小鼠中的肾交感神经活动、压力反射测试、升压测试和高保真ECG。转基因小鼠在翻译自主神经方面越来越重要 在这方面，这种表型小鼠实验室将是必不可少的。在此资助期内，Core将提供类似的生化分析和表型分析服务，但重点是人类数据，包括增强信号处理，自动数据库馈送的数据简化，现有数据库之间的链接，为所有项目开发数据探索工具。

The purpose of the Analytical and Phenotyping Core is to provide state-of-the-art cardiovascular and biochemical analysis of sympathetic baroreflex function, including measurements of sympathetic nerve traffic, catechols, and norepinephrine clearance and spillover. This resource has been critical to the recognition and characterization by our group of new disorders involving the autonomic nervous system, such as dopamine-beta-hydroxylase deficiency. A variety of techniques have been established in this Core to support individual projects, including the determination of plasma volume (Project land 4), heart rate and blood pressure variability (Project 1,2,3, and 4), and baroreflex function (Project 1 and 4). Especially Project 2 among all the others will utilize biochemical evaluation of sympathetic activity which complements other methods of autonomic evaluation detailed in the Clinical Core. Expertise in analysis of ultradian rhythms of cardiovascular parameters for phenotyping will be utilized in Project 1, 3, and 4. Project 1 and 4 will use our novel signal processing tools for neurological signals. In addition, we have implemented an animal phenotyping facility, adapting the methods developed to evaluate autonomic function in humans, to the mice which will be used mainly in Project 1. This includes, among other things, monitoring intra-arterial blood pressure in awake unrestricted mice, monitoring renal sympathetic nerve activity, baroreflex testing, osmopressor tests, and high fidelity ECG, in intact and sinoaortic- denervated mice. Genetically-modified mice are increasingly important in translating autonomic knowledge, and this phenotyping mouse laboratory will be essential in this regard. In this funding period, the Core will provide similar biochemical analytical and phenotyping services but with focus on human data including enhanced signal processing, data reduction with automatic database feeds, links between existing databases, development of data exploration tools for all projects.