Imagining & Image Analysis Core

在过去9年的COBRE赠款支持，爱德华C。卡尔森成像和图像分析核心设施已成为一个重要的研究资源，支持我们的COBRE资助的神经退行性疾病研究小组以及其他研究人员的显微成像和图像分析要求。核心维护和提供荧光，共聚焦，多光子活体和电子显微镜仪器，协助研究人员设计实验，开发新的应用程序，并培训研究人员在显微镜方法和设备的使用，并为核心用户提供与显微镜成像相关的信息资源。核心中的设备包括两个共聚焦显微镜，一个多光子/可见光共聚焦活体显微镜，两个荧光显微镜，两个电子显微镜和一系列辅助显微镜设备。新的先进的共聚焦和多光子设备的收购，维护服务合同，支持工作人员的职位，并增加培训的研究人员在使用我们的成像和图像分析设备提供了研究人员的能力，采用了一系列的成像应用程序之前没有COBRE计划。在核心中进行的工作所产生的出版物数量已经增加到基础科学部门产生的出版物中约有三分之一包括成像核心设施中收集的数据。此外，来自校园其他部分的调查人员，包括生物，化学和化学工程部门以及UND校园的能源和环境研究中心使用该设施。在成像核心设施进行的项目调查了广泛的生物医学问题，包括细胞和组织中的蛋白质分布和表达，蛋白质-蛋白质相互作用，膜受体和转运蛋白的分布和功能，以及作为疾病发病机制标志的细胞和组织变化。继续支持这个核心将是至关重要的，以发展我们的研究企业，并将使核心成为一个可持续的资源，支持研究校园范围内以及该地区的其他地方。

Over the past 9 years of COBRE grant support, the Edward C. Carlson Imaging and Image Analysis Core Facility has become an important research resource that supports the microscopic imaging and image analysis requirements of our COBRE grant supported neurodegenerative disease research group as well as other investigators. The Core maintains and provides access to fluorescence, confocal, multiphoton intravital and electron microscopy instrumentation, assists investigators in the design of experiments, develops new applications, and trains investigators in microscopic methods and use of the equipment, and provides core users with information resources related to microscopic imaging. Equipment housed in the Core includes two confocal microscopes, a multiphoton/visible confocal intravital microscope, two fluorescence microscopes, two electron microscopes, and a range of ancillary microscopy equipment. Acquisition of new sophisticated confocal and multiphoton equipment, maintenance of service contracts, support for staff positions, and increased training of investigators in the use of our imaging and image analysis equipment has provided investigators with the ability to employ a range of imaging applications that were not available prior to the COBRE program. The number of publications arising from work performed in the Core has increased to the point where approximately 1/3 of the publications arising from the basic science departments include data collected in the Imaging Core Facility. Moreover, investigators from other parts of campus including the Departments of Biology, Chemistry, and Chemical Engineering as well as from the Energy and Environmental Research Center on the UND campus use the facility. Projects conducted in the Imaging Core Facility investigate a broad range of biomedical issues including protein distribution and expression in cells and tissues, protein-protein interactions, membrane receptor and transporter distribution and function, and cellular and tissue changes as hallmarks of disease pathogenesis. Continued support of this Core will be critical to growing our research enterprise, and will allow the Core to become a sustainable resource that supports research campus-wide as well as elsewhere in the region.