Flow Cytometry

流式细胞术共享设施自1985年以来一直存在， 为来自癌症中心所有研究项目的许多癌症中心成员提供细胞计数服务。使命 该设施一直服务于ID西蒙癌症中心研究人员的各种研究需求。 我们的目标是继续为癌症中心成员提供具有成本效益的最先进的 技术.在目前的供资期间，平均有一半以上的国际稳定和协调委员会全额供资， 各成员国和准成员国在某一年使用该机制的情况证明了其重要性。流式细胞术 活动目前由三名全职员工进行，他们都是合格的操作员， 接受过广泛的培训。该设施提供咨询、技术咨询和合作。我们 我相信，提供这些服务的集中设施可以促进尖端科学， 一个中央公共区域，供癌症中心研究人员互动和交流科学信息。 自该设施启用以来，已收到许多提供新服务的请求。作为新技术 由于增加了这些服务，该设施提供的服务清单有所增加。人员和仪器 在该设施提供的是能够进行几乎任何流式细胞仪检测要求的癌症 中心调查员。因此，可提供的服务清单包括： 1.多参数（多达9色和10色）免疫荧光分析和细胞分选， 分别 2. DMA含量和细胞周期分析 3.同时免疫荧光和细胞周期分析 4.将单个细胞和少量细胞分选至不同类型的平板/培养瓶中 5.基于细胞在细胞周期中的位置进行细胞分选（批量或单细胞） 6.凋亡分析 7. BrdU掺入 8.活力测定 9.染色体分析（单变量和双变量） 10.受体-配体相互作用 11.动力学分析 12.钙内流分析 13.离线数据分析

The Flow Cytometry shared facility has been in existence since 1985 and has provided essential flow cytometric services to many Cancer Center members from all research programs of the Center. The mission of this facility has been to serve the varied research needs of researchers at the ID Simon Cancer Center. Our goal is to continue to provide Cancer Center members access to cost-effective state-of-the-art technology. The fact that during the current funding period on average more than one-half of all IUSCC full and associate members used the facility in a given year is testimony to its importance. Flow Cytometry activities are currently carried out by three full-time employees, all of whom are well-qualified operators that have received extensive training. The facility provides consultation, technical advice, and collaboration. We believe that a centralized facility providing these services promotes cutting edge science and also serves as a central common area for Cancer Center investigators to interact and exchange scientific information. Since the inception of this facility, there have been many requests for new services. As new techniques were added, the list of services provided by the facility has grown. The personnel and instrumentation available at the facility are capable of performing virtually any flow cytometric assay requested by Cancer Center investigators. The list of available services thus includes: 1. Multiparameter (up to 9- and 10-color) immunofluorescence analysis and cell sorting, respectively 2. DMA content and cell cycle analysis 3. Simultaneous immunofluorescence and cell cycle analysis 4. Sorting of single cells and small number of cells into different types of plates/flasks 5. Cell sorting (bulk or single cells) based on position of cells in cell cycle 6. Apoptosis analysis 7. BrdU incorporation 8. Viability assays 9. Chromosome analysis (univariate and bivariate) 10. Receptor - ligand interactions 11. Kinetic analyses 12. Calcium influx analysis 13. Off-line data analysis