FLOW CYTOMETRY

流式细胞仪共享资源的目标是为用户提供具有成本效益的仪器、专业知识和培训，用于细胞分类和分析。这项技术继续快速发展，特别是随着新型荧光记者的出现、计算能力的增加和更具成本效益的光学设备的出现。 为了满足我们的成员对最先进的流式细胞仪日益增长的需求，DLDCC和BCM管理部门在2007年合作创建了一个全新的流式细胞仪设施。翻修、运营费用和仪器设备得到了170万美元的BCM机构资金和60万美元的DLDCC资金的支持。改造后的设施位于新装修的中央空间，训练有素的用户可以通过钥匙卡每天24小时使用。最先进的仪器，所有的都有 在过去三年中购买的，包括两个满载的荧光激活细胞分选器，三个流动分析仪和一个磁性细胞分离器。该资源由Ellen A.Lumpkin博士和Joel Sederstrom先生领导，前者在流式细胞术方面有9年以上的经验，后者是从大学招聘的。在全国范围内搜索明尼苏达州癌症中心的流式细胞术核心。为确保最佳利用服务，该资源为样品制备、流动分析和细胞分类提供咨询、培训和方案。该资源还配备了两名全职经验丰富的流式细胞仪操作员，他们执行操作员辅助的细胞术，并协助用户进行数据分析。 随着该资源服务和能力的改善，FACS分类在癌症中心成员中增加了500%，FACS分析增加了160%。目前，该资源几乎100%地运行其容量，78%的使用由65名癌症中心调查人员占据，他们的成员涵盖所有科学项目。未来的计划包括进一步扩大服务，招聘一名额外的细胞学家，并在我们的附属机构德克萨斯州儿童医院癌症中心增设第二个站点。

The goal of the Flow Cytometry Shared Resource to provide users with cost-effective instrumentation, expertise and training for cell sorting and analysis. This technology continues to develop at a rapid pace, especially with the advent of novel fluorescent reporters, increased computational capacity and more cost-effective optical equipment. To meet our members' increasing demands for state-of-the-art flow cytometry, the DLDCC and BCM administration collaborated to create an entirely new flow cytometry facility in 2007. Renovation, operating costs and instrumentation has been supported by $1.7 million in BCM institutional funds and >$600,000 in DLDCC funds. The revamped Facility is housed in newly renovated, centrally located space, which is available to trained users 24 h a day via key-card access. State-of-the-art instrumentation, all of which has been purchased in the last three years, includes two fully loaded florescence-activated cell sorters, three flow analyzers and a magnetic cell separator. The Resource is directed by Dr, Ellen A. Lumpkin, who has over nine years of experience in flow cytometry, and Mr. Joel Sederstrom, who was recruited from the Univ. of Minnesota's Cancer Center Flow Cytometry Core in a national search. To ensure optimal use of services, the Resource provides consultations, training and protocols for sample preparation, flow analysis and cell sorting. The Resource is also staffed with two full-time experienced flow cytometrists who perform operator-assisted cytometry, and assist users with data analysis. With the Resource's improved services and capacity, FACS sorting has increased by >500% and FACS analysis has increased 160% among Cancer Center members. At present, the Resource operates near 100% of its capacity, with 78% of usage occupied by 65 Cancer Center investigators whose membership spans all Scientific Programs. Future plans include further expanding services by recruiting an additional cytometrist and by including a second site at our affiliated institution, Texas Children's Hospital Cancer Center.