Cell Morphology and Pathology Core

细胞形态与病理学核心： 细胞形态和病理学核心的目标是为基因治疗中心的研究人员以及对开发囊性纤维化(CF)和其他遗传性疾病的基因转移方法感兴趣的新研究人员提供服务和指导。我们努力在解释疾病病理学方面提供专家协助，因为研究人员在人类疾病的新的大小动物模型中使用基因转移工具。为了实现这些目标，该中心提供：1)对劳动密集型技术的技术援助，如组织或细胞培养切片的准备；2)随时可以获得设备；3)通过集中设备和设施获得经济效益；4)在专门的形态技术和图像分析方法方面提供咨询和指导。这些目标是通过核心主任的监督实现的，核心主任是一位在基因治疗研究中有用的各种显微技术方面经验丰富的CF科学家。 细胞形态和病理学核心的具体目标是： 1)为中心研究人员提供合适的组织固定、加工、冷冻保存、石蜡或塑料包埋、切片和常规染色方法。 2)对于需要检测报告蛋白的调查人员，提供染色技术方面的协助，例如检测和细胞特异性定位(J-半乳糖苷酶活性)。 3)为需要细胞和组织检测特定蛋白质的研究人员提供使用组织化学和荧光技术进行蛋白质抗体标记的帮助。 4)对于需要电子显微镜(电子显微镜或扫描电子显微镜)或共聚焦显微镜的研究人员，提供组织固定、处理以及在照片显微摄影、计算机分析和结果解释方面的协助。 5)对于需要原位杂交的研究人员，在组织制备和探针检测方面提供指导和帮助。 6)为疾病动物模型中的显微标本和大体标本的检查和解释以及它们对基于基因的治疗的反应提供兽医病理学专家支持。 7)协助研究人员开发和使用与基因转移有关的新的和/或专门的形态方法。

Cell Morphology and Pathology Core: The goals of the Cell Morphology and Pathology Core are to provide services and instruction to investigators of the Gene Therapy Center and to new investigators interested in developing gene transfer approaches to cystic fibrosis (CF) and other genetic diseases. We strive to provide expert assistance in the interpretation of disease pathology as investigators use gene transfer tools in new large and small animal models of human disease. To facilitate these goals the Core provides: 1) technical assistance for labor- intensive techniques such as the preparation of sections from tissue or cell cultures, 2) ready access to equipment, 3) economic benefits through centralization of equipment and facilities, and 4) consultation and instruction in specialized morphologic techniques and methods of image analysis. These goals are met through oversight by the Core Director, an established CF scientist with experience in a variety of microscopy techniques that are useful in gene therapy research. The specific aims of the Cell Morphology and Pathology Core are: 1) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding, sectioning and routine staining for Center investigators. 2) For investigators who require detection of reporter proteins, to provide assistance with staining techniques, such as detection and cell-specific localization of (J-galactosidase activity. 3) To provide assistance with antibody labeling of proteins using histochemical and fluorescence techniques to investigators who require cell and tissue detection of specific proteins. 4) For investigators who require electron microscopy (TEM or SEM) or confocal microscopy, to provide tissue fixation, processing, and assistance in photo micrography, computerized analysis and interpretation of results. 5) For investigators who require in situ hybridization, to provide instruction and assistance in tissue preparation and detection of the probe. 6) To provide expert veterinary pathology support for the examination and interpretation of microscopic to gross specimens in animal models of disease and their response to gene-based therapies. 7) To provide assistance to investigators in the development and use of new and/or specialized morphological methods relevant to gene transfer.