A Novel Multilayered Membrane for Periodontal Regeneration

描述（由申请人提供）：随着美国人口老龄化，重要的是找到能够促进牙周再生的新型牙科生物材料，并将其转化为临床实践。作为牙医和材料科学家，申请人能够弥合生物材料开发之间的差距。以及它们在使用纳米技术、组织工程和临床知识的患者护理中的应用。该奖项的目的是为主要研究者提供细胞和分子生物学，骨骼生物学和转化研究方面的广泛培训，重点是牙周组织再生。这项应用中的实验旨在纳入新的方法，并将由一个多学科专家小组指导，他们将提供所需的培训，以提高Bottino博士的科学技术和分析技能。主要研究者的最终目标是成为一个富有成效的和独立的临床科学家谁可以确保联邦资金赞助一个独立的研究计划。拟议研究的中心假设是，通过添加磷酸钙和成纤维细胞生长因子-2（CaP/FGF 2）和FGF 2，能够以位置特异性方式刺激各种组织类型再生的多层膜将导致适当位置的牙槽和牙周组织再生。文献表明，没有一种单一的可植入生物材料可以始终如一地指导多种组织类型的协调生长和发育，特别是在非常大的牙周缺损中。因此，迫切需要开发再生疗法 促进口腔和颅面组织再生和重建。所提出的研究的具体目的是（1）使用体外测定模型来最大化多层种植体表面层的组织特异性再生能力，（2）使用体内犬模型来评估CaP/FGF 2对牙槽骨缺损中的骨再生的有效性，和（3）使用体内犬模型以特定位置的方式评估多层植入物再生软组织和矿化组织的有效性。主要研究者制定了一个全面的职业发展计划，其中包括正式的课程，实验室指导，以涵盖密集的细胞和分子生物学培训，以及在他的指导团队的监督下结合各种骨/牙周组织再生相关分析的体内技术。总的来说，K 08奖有能力提供所需的资源和培训，为申请人提供基础和关键的理论和实践手段，使其成为一名高素质的临床科学家，能够将其生物材料知识转化为临床实践。

DESCRIPTION (provided by applicant): With the US population aging, it is important to find novel dental biomaterials that will promote periodontal regeneration, and translate these to clinical practice. As both a dentist and materials scientist, the applicant is in a position to brige the gap between biomaterials development. and their application in patient care using nanotechnology, tissue engineering and clinical knowledge. The purpose of this award is to provide the principal investigator extensive training in cellular and molecular biology, skeletal biology, and translational research with an emphasis on periodontal tissue regeneration. The experiments in this application have been designed to incorporate new methodologies, and will be guided by a multidisciplinary team of experts who will provide the required training to advance Dr. Bottino's scientific technical and analytic skills. The principal investigator's ultimae goal is to become a productive and independent clinician-scientist who can secure federal funding to sponsor an independent research program. The central hypothesis of the proposed research is that a multilayer membrane capable of stimulating the regeneration of various tissue types in a location specific manner, through the addition of calcium phosphate and fibroblast growth factor-2 (CaP/FGF2) and FGF2, will lead to alveolar and periodontal tissue regeneration in the appropriate locations. The literature indicates that no single implantable biomaterial can consistently guide the coordinated growth and development of multiple tissue types, especially in very large periodontal defects. Thus, there is a critical need to develop regenerative therapies to promote oral and craniofacial tissue regeneration and reconstruction. The specific aims of the proposed research are (1) To use an in vitro assay model to maximize the tissue-specific regenerative capacity of the multilayered implant surface layers, (2) To assess the effectiveness of CaP/FGF2 on bone regeneration in an alveolar defect using an in vivo canine model, and (3) To assess the effectiveness of the multilayered implant to regenerate soft and mineralized tissues in a location-specific manner using an in vivo canine model. The principal investigator has devised a comprehensive career development plan that includes formal coursework, laboratory guidance to cover intensive cell and molecular biology training, as well as in vivo techniques combined with a diverse set of bone/periodontal tissue regeneration related analyses under the supervision of his mentoring team. Collectively, the K08 award has the capacity to provide the resources and training needed to give the applicant the foundation and critical theoretical and practical means to become a highly qualified clinician-scientist who can translate his knowledge of biomaterials to clinical practice.