Reactive Oxygen Species Core

核心C的目的是为PPG的参与者提供对细胞、组织和膜中氧化还原敏感蛋白的活性氧簇(ROS)、还原型谷胱甘肽(GSH)、谷胱甘肽二硫化物(GSSG)、半胱氨酸残基的氧化修饰的最先进的测量。Core C将协助准备和处理各种生物样品，以便使用电子自旋共振(ESR)光谱、高效液相色谱(HPLC)和纳米LC MS/MS分析进行分析。此核心设施已存在 自1999年以来一直支持血管细胞和组织中的超氧阴离子(O2*)、过氧化氢(H2O2)和一氧化氮(NO)的研究，是哈里森博士、格里德林博士、泰勒博士和乔博士先前项目研究的重要组成部分(1-10)。这一设施将大大加强各个实验室的工作。 并将加强PPG参与者之间的合作

The purpose of Core C is to provide state-of-the-art measurements of reactive oxygen species (ROS), reduced glutathione (GSH), glutathione disulfide (GSSG), oxidative modifications of cysteine residues of redox-sensitive proteins in cells, tissues and membranes for participants of the PPG. Core C will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy, High Perfonnance Liquid Chromatography (HPLC) and nano-LC MS/MS analysis. This Core facility has existed since 1999 and has supported numerous studies of superoxide (O2*), hydrogen peroxide (H2O2) and NO in vascular cells and tissue, and it was an essential part of the previous program project studies by Drs. Harrison, Griendling, Taylor and Jo (1-10). This facility will significantly enhance the work of the individual laboratories. and will enhance collaborations between participants of the PPG