Vector Core

在中心的当前周期中，向量核心经历了实质性的演变，以利用 在新型病毒载体方面已经取得了巨大的进展。朱莉·约翰斯顿博士，向量核心主任 以她在DNA和RNA病毒载体方面的丰富经验监督核心。在过去的四年里， 向量核心提供的服务列表呈指数级增长。在上一次的时候 续期赠款核心提供了基于腺病毒血清型5型腺相关病毒载体的服务 病毒(AAV)2型和小鼠为基础的生态型和两性型逆转录病毒。目前 核心提供的服务清单已经扩大到包括：腺病毒-基于人类的载体 Ad5和至少5个猿腺病毒与更多新的猿腺病毒分离；AAV载体 基于人类AAV血清型1、2和5型以及猿猴AAV血清型7和8型，该列表扩展到 在下一个拨款周期中包括10种新的AAV血清型；慢病毒-至少6种不同的 基于HIV和FIV的伪型；以及具有各种伪型的小鼠逆转录病毒。除了这一点， 病媒生物产量的增加保证这些病媒生物的质量是非常必要的。目前有一种质量 使用载体核心的对照组，以检查在本单位制造的任何载体准备的身份、效力和纯度。

The Vector Core has undergone substantial evolution during the current cycle of the Center to leverage the tremendous progress that has been made in novel viral vectors. Dr. Julie Johnston, Director of Vector Core oversees the core with her tremendous experience in DNA and RNA viral Vectors. During the last four years, the list of services provided from the Vector Core has expanded exponentially. At the time of the last renewal of the grant the core provided services with vectors based on adenovirus serotype 5, adenoassociated virus (AAV) serotype 2 and murine based ecotropic and amphotropic retroviruses only. Currently the list of services provided by the core has expanded to include: Adenovirus - vectors based on human Ad5 and at least 5 simian adenoviruses with more novel simian adenoviruses in isolation; AAV- vectors based on the human AAV serotypes 1,2 and 5 and simian AAV serotype 7 and 8 with this list expanding to include 10 additional novel AAV serotypes over the next cycle of the grant; lentiviruses - at least 6 different pseudotypes based on HIV and FIV; and murine retroviruses with various pseudotypes. In addition to this increase in vector production it was very essential to quality assure these vectors. Currently there is a quality control group with Vector Core to check identity, potency and purity of any vector prep made in this unit.