600 MHZ NMR RF Console with 19F NMR Probe

7.项目总结 这项提议旨在为最先进的600 MHz核磁共振射频(RF)控制台提供资金，配备19F/1H (13C，15N，31P)三重共振(TR)核磁共振探头，具有生物溶液核磁共振能力。主要需求 更换核磁共振控制台的目的是解决600 MHz核磁共振数据收集的有限容量问题 在罗格斯大学、新泽西州立大学和罗伯特大学广泛且不断增长的核磁共振用户群中 新泽西医学与牙科大学伍德·约翰逊医学院(RWJMS)。 射频控制台将取代16年的旧瓦里安INOVA 600 MHz核磁共振控制台，其功能仅限于 进行我们日益壮大的NIH资助的核磁共振用户社区所需的现代核磁共振实验。这个 更换射频控制台和探头，以及现有的牛津磁铁和低温TR-核磁共振探头 已经安装的，形成了一个集成的生物分子核磁共振系统。这份建议书是为 在将使用拟议的核磁共振仪器的大约24个研究实验室中，包括主要用户， 次要用户和协作者。由于以下设备的可用性有限，此旧主机无法持续维护 更换部件，以及无法运行现代核磁共振数据收集软件。主要用户组包括 (I)先进生物技术和医学中心(CABM)，(Ii)纽约癌症研究所 泽西岛(CINJ)，(三)东北结构基因组学联盟(NESG)，和(四)外部用户 组。CABM是一个跨机构的研究机构，由RWJMS和罗格斯大学联合管理 大学。它是整个生命科学研究界的科学互动中心。CABM 包括一个由13名常驻首席调查员组成的小组，他们在以下广泛领域开展研究计划 癌症、传染病和神经发育/神经退行性疾病。CINJ是一家NIH- 被指定为P30综合癌症中心，约有60名居民和准成员参与。 活动跨越基础研究和药物发现，通过转译研究、临床 研究和癌症治疗。第三个用户组是NESG联合体，由蛋白质结构提供资金 国家普通医学科学研究院(PSI-生物学)倡议。Nesg是四大- 蛋白质结构生产规模中心，支持结构生物学领域的广泛合作， 细胞生物学、癌症生物学、蛋白质样品制作和技术开发。第四组用户 包括其他大学的教职员工和学生，他们利用组织良好的数据收集和 CABM生物分子核磁共振设施的数据分析设施。这些研究人员跨越了有限的群体 蛋白质核磁共振方面的专业知识，将样品和/或学生送到设施进行数据收集和分析 努力，以经验丰富的核磁共振光谱仪谁需要访问高场核磁共振数据收集。 1

7. Project Summary This proposal seeks to fund a state-of-the-art 600 MHz NMR radio frequency (RF) console, with 19F/1H (13C,15N,31P) triple resonance (TR) NMR probe, with capabilities for biological solution NMR. The primary need for this replacement NMR console is to address the limited capacity for NMR data collection at 600 MHz among a broad and growing NMR user group at Rutgers, The State University of New Jersey, and The Robert Wood Johnson Medical School (RWJMS) of the University of Medicine and Dentistry of New Jersey (UMDNJ). The RF console will replace a 16-Yr old Varian INOVA 600 MHz NMR console that has limited capabilities to carry out modern NMR experiments required by our growing NIH-funded NMR user community. The replacement RF console and probe, together with an existing Oxford magnet and cryogenic TR-NMR probe that are already installed, form an integrated biomolecular NMR system. This proposal was prepared on behalf of some 24 research laboratories that will utilize the proposed NMR instrumentation, including major users, minor users, and collaborators. This old console cannot be sustainably maintained due to limited availability of replacement parts and its inability to run modern NMR data collection software. The principal user groups are (i) The Center for Advanced Biotechnology and Medicine (CABM), (ii) The Cancer Institute of New Jersey (CINJ), (iii) The Northeast Structural Genomics Consortium (NESG), and (iv) External Users Group. The CABM is an inter-institutional research institute that is jointly administered by RWJMS and Rutgers University. It serves as hub for scientific interactions throughout the life science research community. CABM includes a group of 13 resident principal investigators who conduct research programs in the broad areas of cancer, infectious disease, and neurodevelopment / neurodegenerative disorders. The CINJ is an NIH- designated P30 Comprehensive Cancer Center, involving some 60 Resident and Associate members. Activities span basic research and pharmaceutical drug discovery, through translational research, clinical research, and cancer treatment. The third user group is the NESG Consortium, funded by the Protein Structure Initiative (PSI-Biology) of the National Institutes of General Medical Sciences. The NESG is one of four Large- scale Centers for Protein Structure Production, supporting a wide range of collaborations in structural biology, cell biology, cancer biology, protein sample production, and technology development. A fourth group of users comprises faculty and students at other universities who take advantage of well-organized data collection and data analysis facilities of the CABM Biomolecular NMR facility. These researchers span groups with limited expertise in protein NMR who send samples and/or students to the facility for data collection and analysis efforts, to experienced established NMR spectroscopists who need access to high field NMR data collection. 1