GEISHA: A Chicken Embryo Gene Expression Resource

描述（由申请人提供）：生物医学研究的一个核心范例是，了解基因功能将最终改善人类健康。关键的第一步是了解基因在胚胎发生和成年期间何时何地表达。鸡胚作为实验模式生物具有与人类惊人的相似性、低成本和历史悠久等优点，可用于大规模的基因表达筛选。此次更新申请的目的是继续支持和扩展GEISHA （gallus expression in situ hybridization analysis）基因表达数据库，这是一个已建立的鸡胚原位杂交基因表达信息和相关基因组元数据的社区资源。该应用程序的具体目的是：(1)用高通量原位杂交筛选、科学文献和其他研究人员提供的鸡基因表达信息填充GEISHA数据库。将进行高通量原位杂交分析，以确定发育调节基因的表达模式。原位杂交图像和相关信息也将从出版物和研究人员获得。2)扩大艺伎数据库，纳入新的信息类型和特征。原位杂交图像和相关数据将与其他基因表达项目交换，以便在每个平台上本地显示跨物种的表达信息。此外，还将增加BioMart数据整合系统和出版物管理系统，以提高检索能力，并展示与基因相关的出版物。3)用户界面将得到增强，以适应新的功能，并方便导航。基因表达报告页面将被重组和扩展，包括详细的基因摘要信息、基因相关出版物和多物种的基因表达信息。用户友好的查询功能将扩展到包括OMIM人类疾病基因和基因本体术语的搜索。通过这些改进，GEISHA将继续为生物医学研究提供有价值和全面的资源。

DESCRIPTION (Provided by Applicant): A central paradigm of biomedical research is that understanding gene function will ultimately lead to improvements in human health. A crucial first step is to know where and when genes are expressed during embryogenesis and in the adult. The chicken embryo provides many advantages for large-scale expression screening, including striking similarities to human, low expense, and a long history as an experimental model organism. The goal of this renewal application is to continue support and expansion of the GEISHA (gallus expression in situ hybridization analysis) gene expression database, an established community resource for chicken embryo in situ hybridization gene expression information and associated genomic metadata. Specific aims of this application are (1) to populate the GEISHA database with chicken gene expression information arising from targeted high throughput in situ hybridization screens, from the scientific literature, and from other researchers. High throughput in situ hybridization analyses will be performed to determine the expression patterns of developmental regulatory genes. In situ hybridization images and associated information will also be curated from publications and obtained from researchers. 2) The GEISHA database will be expanded to encompass new types of information and features. In situ hybridization images and associated data will be exchanged with other gene expression projects to enable cross-species display of expression information locally on each platform. The BioMart data integration system and a publication management system will also be added to enhance search and retrieval capabilities and to display gene related publications. 3) The user interface will be enhanced to accommodate new features and to facilitate navigation. Gene Expression Report pages will be reorganized and expanded to include detailed gene summary information, gene-related publications, and gene expression information for multiple species. User-friendly query features will be expanded to include searching by OMIM human disease gene and Gene Ontology terms. Through these enhancements, GEISHA will continue to provide a valuable and comprehensive resource for biomedical research. RELEVANCE: The objective of this project is to enhance an important existing gene expression resource for the chicken embryo, a recognized biomedical model of human disease. These services will provide valuable information that will assist efforts across a wide variety of related fields towards the identification of mechanisms underlying human diseases.