Viral vector Core

病毒载体核心是宾夕法尼亚大学糖尿病研究中心（DRC）成员对使用病毒载体进行基因治疗和基础研究应用感兴趣的重要资源。载体核心提供腺病毒载体、腺相关病毒载体（AAV）和慢病毒载体，并提供包括克隆、DNA扩增和表征、载体设计和咨询在内的全方位服务。该中心位于该国首屈一指的基因治疗研究实验室之一，专门从事新型AAV血清型载体和假型慢病毒载体的生产和分销。刚果民主共和国病媒核心的目标如下：利用最先进的设施和敬业的专业人员，核心将：(1)为刚果民主共和国中心成员提供用于糖尿病和相关内分泌疾病应用的先进病媒技术；(2)与刚果民主共和国执行委员会成员合作，开发专门供刚果民主共和国成员使用的载体或重组病毒；(3)将宾夕法尼亚大学实验室开发的具有优越转导谱的新创建载体转移到核心，以进行验证并分发给DRC成员；(4)就病媒构建和实验设计向刚果民主共和国成员提供咨询；(5)开发一个基于网络的界面，配合最近开发的病媒核心数据库，使刚果民主共和国成员能够请求服务，监测病媒生产状况，并访问病媒核心为其实验室生产的所有病媒的历史数据。DRC载体核心的总体目标，就像更大的宾夕法尼亚大学DRC一样，是为宾夕法尼亚大学和周边机构的研究人员提供服务，并帮助推进糖尿病相关研究，以实现预防、治疗和治愈这种毁灭性疾病的目标。

The Viral Vector Core is a critical resource for Penn Diabetes Research Center (DRC) members interested in the use of viral vectors for gene therapy and basic research applications. The Vector core provides adenoviral vectors, adeno-associated viral vectors (AAV) and lentiviral vectors and offers a full range of services including cloning, DNA amplification and characterization, vector design and consultation. The Core is located within one of the premier gene therapy research laboratories in the country and specializes in the production and distribution of novel AAV serotype vectors and pseudotyped lentiviral vectors. The objectives of the DRC Vector Core are as follows: Using state-of-the-art facilities and dedicated professional staff, the Core will: (1) provide DRC Center members with access to advanced vector technologies for use in diabetes and related endocrine disorder applications; (2) work with DRC executive committee members to develop vectors or recombinant viruses specifically for use by DRC members; (3) transition newly created vectors with superior transduction profiles developed in Penn laboratories to the Core for validation and distribution to DRC members; (4) provide consultation to DRC members for vector construction and experimental design and (5) develop a web-based interface to accompany the recently developed Vector Core database to enable DRC members to request services, monitor the status of vector production and access historical data on all vectors produced for their laboratory by the Vector Core. The overall goal of the DRC Vector Core, like that of the larger Penn DRC, is to serve investigators at Penn and surrounding institutions, and aid in the advancement of diabetes-related research toward the goal of prevention, treatment, and cures for this devastating disease.