Core 3 - Integrative Cell Phenotyping and Morphology Core

几种细胞类型参与了风湿性疾病的炎症过程。要了解这些疾病的病理生物学，通常需要剖析特定细胞亚群的作用。综合细胞表型和形态核心(ICPMC)将以合理的成本为辛德纳提风湿病核心中心(CRDCC)研究基地的研究人员提供技术专业知识、援助和设备，这些研究需要单细胞分析。 ICPMC将提供关于正确使用和解释一些术语的培训 免疫表型方法，包括流式细胞术、免疫组织化学、原位杂交和组织学。核心人员将在以下领域提供专门知识和协助： *细胞表面表型分析(基于流动和基于病理) *形态细胞表型分析(基于流动和基于病理) *细胞内细胞因子分析(基于流量和基于病理学) *细胞增殖分析(基于流) *细胞周期和DNA指数分析(基于流量) *细胞凋亡分析(基于流量和基于病理) *基因表达分析(基于流和基于病理) *钙流量分析(基于流量) *单元格排序(基于流) *抗原特异性T细胞分析(基于流量) 此外，ICPMC将促进免疫表型数据的获取、分析和存储，维持免疫表型程序的标准和质量控制，并协助应用和开发新技术。

Several cell types are involved in inflammatory processes in rheumatic diseases. Understanding the pathobiology of these diseases often requires dissecting out contributions of spedfic cell subpopulations. The Integrative Cell Phenotyping and Morphology Core (ICPMC) will provide technical expertise, assistance and equipment at a reasonable cost to investigators in the Research Base of the Cindnnati Rheumatic Disease Core Center (CRDCC) whose studies require single cell analysis. The ICPMC will provide training in the proper use and interpretation of a number of immunophenotyping methodologies, including flow cytometry, immunohistochemistry, in situ hybridization cind histology. Expertise and assistance will be provided by Core personnel in the following areas: * Cell surface phenotype analysis (flow-based and pathology-based) * Morphological cell phenotype analysis (flow-based and pathology-based) * Intracellular cytokine analysis (flow-based, and pathology-based) * Cell proliferation analysis (flow-based) * Cell cycle and DNA index analysis (flow-based) * Cell apoptosis analysis (flow-based and pathology-based) * Gene expression analysis (flow-based and pathology-based) * Calcium flux analysis (flow-based) * Cell sorting (flow-based) * Antigen-specific T cell analysis (flow-based) In addition, the ICPMC will facilitate acquisition, analysis and storage of immunophenotyping data, maintain standards and quality control for immunophenotyping procedures, and assist in the application and development of new techruques.