Microscopy and Imaging Center

总结 成像技术构成了关键的工具，带来了承担的创造力和权力 在现代肾脏研究的许多方面。参与这项研究的肾脏研究人员 计划项目补助金需要随时获得高质量的成像设备和服务。 现有的显微镜和成像中心核心设施的设计，以满足这一需求。 该核心的结构是将来自几个国家的资源和专门知识结合在一起， 将大学内的不同地点整合成一个统一的实体， 设计和执行成像研究的合作伙伴。该设施包括全方位的高 质量成像设备，包括：荧光显微镜，荧光成像 系统，一种新的激光扫描共聚焦显微镜，旋转圆盘共聚焦显微镜， 电子显微镜和许多用于组织切片和样品制备的装置。的 核心技术人员在成像方法学方面具有数十年的综合经验 和技术开发。监督人员包括两名资深科学家，他们都是 在各种成像模式应用于肾脏方面具有广泛的专业知识， 生理学研究该团队将共同努力实现以下目标：1） 为PPG集团研究人员提供高质量成像模式的便捷途径，包括 荧光显微镜，激光扫描共聚焦显微镜，真实的时间成像， 细胞和小管的生理参数和电子显微镜; 2）提供PPG组 具有光学和电子显微镜样品制备和处理专家的研究人员; 3） 为PPG集团研究人员提供生成、解释和 静态图像的定量，包括记录器官结构、肾组织学和 肾蛋白的亚细胞定位;以及4）为PPG组研究人员提供 获得制作活细胞动态图像所需的设备和专业知识， 组织，包括器官结构的体内评估，细胞内离子的测量 浓度和蛋白质运输的真实的时间观察。

Summary Imaging techniques constitute critical tools that are brought to bear with creativity and power in many aspects of modern renal research. The renal researchers who participate in this Program Project Grant require ready access to high quality imaging equipment and services. The existing Microscopy and Imaging Center core facility has been designed to meet this need. The core has been structured in a manner that unites resources and expertise from several different locations within the University into a single unified entity that can serve as an integral partner in the design and execution of imaging studies. The facility includes a full range of high quality imaging equipment, including: fluorescence photomicroscopes, fluorescence imaging systems, a new laser scanning confocal microscope, a spinning disk confocal microscope, an electron microscope and numerous devices for tissue sectioning and sample preparation. The technical staff of the core manifests decades of combined experience in imaging methodologies and technique development. The supervisory staff includes two senior scientists, both of whom have extensive expertise in the application of the various modalities of imaging to renal and physiological research. This team will work together to meet the following objectives: 1) to provide PPG group researchers with ready access to high quality imaging modalities, including fluorescence microscopy, laser scanning confocal microscopy, real time imagining of physiological parameters in cells and tubules and electron microscopy; 2) to provide PPG group researchers with expert sample preparation and processing for light and electron microscopy; 3) to provide PPG group researchers with expert assistance in the generation, interpretation and quantitation of static images, including those documenting organ structure, renal histology, and the subcellular localizations of renal proteins; and 4) to provide PPG group researchers with access to the equipment and expertise required to produce dynamic images of living cells and tissues, including in vivo assessment of organ structure, measurements of intracellular ion concentrations, and real time observations of protein trafficking.