AB/SCIEX QTrap 6500 Mass Spectrometer Metabolic profiling system

描述（由申请人提供）：本提案旨在寻求资金，用于购买混合三重四极杆/线性离子阱（AB Sciex QTRAP 6500）质谱代谢组学分析系统，该系统具有多路复用MPX 2 HPLC接口。该仪器将扩展和升级俄亥俄州东北部地区的质谱分析能力，推进克利夫兰诊所基金会（CCF）及其合作机构、凯斯西储大学（CWRU）、克利夫兰CTSA其他成员机构和克利夫兰州立大学（CSU）的研究计划。主要用户都有NIH资助的研究经费，包括最近的多个计划项目赠款和R 01，所有这些项目和研究目前都使用质谱法进行询问，调查和回答有关影响人类健康的重大疾病的关键问题。由于充分利用了该区域现有的仪器，许多调查员现在必须提前数月安排时间，以获得这一技术和/或前往遥远的机构。如申请中所述，支持的项目主要包括生物标志物发现和临床/转化研究，几乎所有这些研究都利用了NCRR资助的克利夫兰CTSA。所要求的质谱仪具有高通量能力，可确保及时使用这类仪器，同时又不牺牲这些项目所需的多功能性、灵敏度和特异性。这项分析活动将在我们现有的小分子质谱核心内进行，该核心通过进行结构解析研究、生物标志物定量、蛋白质测序/鉴定和相关服务，为CCF、CWRU、CSU和邻近机构的研究人员提供支持。质谱仪越来越多地被CCF和伙伴机构调查人员用作分析工具，需求大大超过可用性，导致访问严重延迟，并阻碍了生产力。获得所要求的质谱仪对于缓解等待分析的样品积压至关重要。CCF已投入大量资源用于该地区的质谱发展，包括为小分子质谱核心实验室空间的定制翻新，监督其运作的人员，以及确保其长期运作和维护的财政计划。

DESCRIPTION (provided by applicant): The purpose of this proposal is to seek funds for the purchase of a hybrid triple quadrupole/linear ion trap (AB Sciex QTRAP 6500) mass spectrometry metabolomics profiling system with a multiplexed MPX2 HPLC interface. This instrument will expand and upgrade mass spectrometry capabilities within the Northeastern Ohio region, advancing research programs within the Cleveland Clinic Foundation (CCF) and its partner institution, Case Western Reserve University (CWRU) other Cleveland CTSA member institutions and Cleveland State University (CSU). Major Users all have NIH-funded research grants, including recent multiple Program Project Grants and R01's, all which have Projects and studies that are currently conducted using mass spectrometry to ask, investigate and answer key questions concerning major diseases that affect human health. Due to the full utilization of available instrumentation in the region, many Investigators now have to schedule time many months in advance to obtain access to this technology and/or travel to distant institutions. As detailed in the application, supported projects primarily include biomarker discovery and clinical/translational studies nearly all of which also utilize the NCRR-funded Cleveland CTSA. The requested mass spectrometer provides the high through-put capabilities that will assure timely access to this type of instrumentation, while not sacrificing versatility, sensitivity and specificity required for these projects. This analytical activity will be conducted within our existing Small Molecule Mass Spectrometry Core, which provides support to investigators at CCF, CWRU, CSU and neighboring institutions by performing structural elucidation studies, biomarker quantification, protein sequencing/identification, and related services. Mass spectrometry is increasingly being used as an analytical tool by CCF and partner institution investigators, with demand greatly outpacing availability, resulting in significant delays in access, and hampered productivity. The acquisition of the requested mass spectrometer is essential to alleviate the backlog of samples waiting to be analyzed. CCF has committed substantial resources to the development of mass spectrometry in the region, including custom renovation of laboratory space for Small Molecule Mass Spectrometry Core, personnel to oversee its operation, and a fiscal plan to insure its operation and maintenance for the long-term.