Xenbase: a Xenopus Model Organism Database

动物模型提供了强有力的实验工具来测试基因功能和询问生物学特性。 途径。人类和模式生物之间的比较功能基因组学已经导致了丰富的 很明显，这将继续发挥重要作用，释放潜力， 人类健康的基因组。在这个后基因组时代，高度注释和容易获得的数据是 研究人员越来越需要整合大量的序列、表达和功能数据， 转化为有意义的生物合成本提案的目标是维护和增强Xenbase; 非洲爪蟾模式生物数据库。非洲蛙非洲爪蟾长期以来一直是 细胞和发育生物学家使用的生物，提供了许多基本的见解。大 丰富的非洲爪蟾胚胎使复杂的胚胎学成为可能， 通过显微注射和生化分析。再加上基因组和EST的最新进展 测序，全长cDNA集的产生，微阵列分析，功能基因组学筛选，基因 使用反义吗啉代寡核苷酸敲除，快速转基因的发展和建立 Xenbase的开发使Xenopus成为功能基因组学的强大系统。Xenbase是一个全面的 一个数据库，提供了一个门户网站，以相互关联的爪蟾数据，包括基因组，mRNA和蛋白质 序列，基因表达，功能和出版物，这一切都是高度整合与NCBI和其他 模式生物数据库。本提案的目标是维护和扩展Xenbase。目标1）保持 并通过实施TestBase数据挖掘和手动策展来进一步策展Xenbase中的数据。目的 2)通过引入吗啉代和突变表型增强Xenbase功能。目标3）支持新的 Xenbase基因页面上的内容，包括mRNA剪接微阵列和Xenopus WIKI。目标4） 通过进一步将Xenbase数据与 NCBI和其他模式生物数据库。这项提案将授权非洲爪蟾研究，并提供 更广泛的科学界获得丰富的爪蟾数据的一种手段。

Animal models provide powerful experimental tools to test gene function and interrogate biological pathways. Comparative functional genomics between humans and model organisms has led to a wealth of discoveries, and it is clear that this will continue to play an important role in unlocking the potential of the genome for human health. In this post genomic era highly annotated and easily accessible data are increasingly essential for researchers to integrate vast amounts of sequence, expression and functional data into a meaningful biological synthesis. The goal of this proposal is to maintain and enhance Xenbase; the Xenopus model organism database. The African frogs Xenopus has long been one of the premiere model organisms used by cell and developmental biologists, providing numerous fundamental insights. The large and abundant Xenopus embryos enable sophisticated embryology, easy manipulation of gene expression by microinjection and biochemical analyses. Together with recent advances in genome and EST sequencing, the generation of full-length cDNA sets, microarray analysis, functional genomics screens, gene knockdown using anitsense morpholino oligos, the development of rapid transgenics and the establishment of Xenbase has made Xenopus a powerful system for functional genomics. Xenbase is a comprehensive database that provides a portal to inter-related Xenopus data including genomic, mRNA and protein sequence, gene expression, function and publications, which is all highly integrated with NCBI and other model organism databases. The goal of this proposal is to maintain and expand Xenbase. Aim 1) Maintain and further curate the data in Xenbase by implementing Testpresso data mining and manual curation. Aim 2) Enhance Xenbase functionality by introducing morpholino and mutant phenotypes. Aim 3) Support new content on Xenbase gene pages including mRNA splicing microarrays, and a Xenopus WIKI. Aim 4) Continue and expand collaborative research and service efforts by further integrating Xenbase data with NCBI and other model organism data bases. This proposal will empower Xenopus research and provide the broader scientific community a means of accessing a wealth of Xenopus data.