Broad wavelength range Zeiss 780 NLO/confocal system for the Cornell Imaging Core

描述(由申请人提供)：这份高端的S10计划申请资金，从Newport/Spectra-物理公司(The Insight激光器)购买蔡司LSM780NLO多光子/单光子共焦显微镜，该显微镜与宽调谐飞秒激光器耦合。该仪器将安置在康奈尔大学伊萨卡校区威尔大厅地下室的康奈尔生物技术资源中心(BRC)成像核心设施中。其目标是提供一种现代的、用户友好的组合非线性和共聚焦荧光成像系统，供位于康奈尔校园的NIH资助的10个主要用户和6个次要用户使用。该仪器将取代过时的Bio-Rad 1024多光子系统，该系统最初于1996年与多光子显微镜的最初许可证获得者Bio-Rad合作安装在P41发展资源(DRBIO)中。该仪器后来被康奈尔生物技术资源中心(BRC)的成像设施接管，该设施为整个校园提供服务。它经过了多次改装，但仍在废弃的IBM OS/2操作系统和Bio-Rad LaserSharp软件包下运行，使其完全不受支持。收购所要求的最先进的系统将极大地提高康奈尔成像设备的能力，并使我们能够更好地服务于我们NIH资助的研究人员的需求。这台仪器不仅将取代不受支持的16年前的多光子显微镜，还将增加几个以前无法使用的新功能，这些功能将对我们的 用户。其中包括高达1300 nm的双光子激发波长，这将以更高的可行性提供更深层次的成像，并首次允许我们的用户评估目前我们的标准钛宝石光源无法访问的红色染料和荧光蛋白家族。该系统还拥有最新的最高量子效率探测器--包括共焦头和外部非扫描探测器系统。所有被提议的显微镜的用户都将对活体动物、组织或组织制剂进行成像，所有人都将受益于这些新的光电探测器提供的2到3倍的荧光检测能力。同样重要的是，蔡司系统和Zen软件根据我们的用户对3D局部光激活、光漂白和靶向光消融的需求，提供了极其精确的激光激发控制。所要求的仪器是专门为我们NIH资助的主要和次要用户的需求量身定做的，并将在很长一段时间内满足他们的荧光成像需求。

DESCRIPTION (provided by applicant): This high-end S10 proposal requests funds to purchase a Zeiss LSM780 NLO multiphoton/single-photon confocal microscope coupled to a broad tuning femtosecond laser from Newport/Spectra-Physics (the Insight laser). The instrument will be housed in the Cornell Biotechnology Resource Center's (BRC) Imaging Core facility in the basement of Weill Hall on the Ithaca campus of Cornell University. The goal is to provide a modern, user-friendly combined nonlinear and confocal fluorescence imaging system for use by ten NIH-funded major users and 6 minor users located on the Cornell campus. The instrument will replace an outdated Bio-Rad 1024 multiphoton system which was installed originally in 1996 in the P41 Developmental Resource (DRBIO) in collaboration with Bio-Rad, the initial licensee of multiphoton microscopy. The instrument was later taken over by the Cornell Biotechnology Resource Center (BRC) imaging facility which serves the entire campus. It has been retrofitted numerous times, but still runs under the defunct IBM OS/2 operating system and the Bio-Rad LaserSharp software package rendering it completely un-supportable. Acquisition of the state-of-art system requested will greatly advance the capabilities of the Cornell Imaging facility, and allow us to better serve the needs of our NIH funded researchers. This instrument will not only be replacing an unsupportable 16 year old multiphoton microscope, but will be adding several new previously unavailable features that will be of great benefit to our users. These include two-photon excitation wavelengths up to 1300 nm that will provide for deeper imaging with higher viability, and, for the first time, allow our users to assess families o red dyes and fluorescent proteins that are currently inaccessible with our standard Ti:Sapphire source. The system also has the most up-to-date highest quantum efficiency detectors - both in the confocal head and the external non-descanned detector system. All of the users of this proposed microscope will be imaging live animals, tissues or tissue preparations and all will benefit from the 2 to 3 fold increase in fluorescence detection these newer photodetectors provide. Equally important, the Zeiss system and Zen software provide extremely precise control of the laser excitation as required by our users who have a need for 3D localized photoactivation, photobleaching and targeted photoablation. The requested instrument is specifically tailored to the needs of our NIH funded major and minor users, and will provide for their fluorescence imaging needs well into the future.